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題名 | 毛髮和精液於豬緊迫敏感症基因型簡易檢測之應用=Simplified Protocol for Genotyping Porcine Stress Syndrome Using Hair Roots and Semen |
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作者 | 劉名允; 鄭益謙; 楊平政; | 書刊名 | 中華民國獸醫學會雜誌 |
卷期 | 25:4 1999.12[民88.12] |
頁次 | 頁316-319 |
分類號 | 437.657 |
關鍵詞 | 毛髮; 精液; 豬緊迫敏感症基因型; Hair; Semen; PSS genotype; |
語文 | 中文(Chinese) |
英文摘要 | The genotypes of Porcine Stress Syndrome (PSS) can be detected by using the Polymerase Chain Reaction (PCR) to amplify a fragment with point mutation of ryanodine receptor gene, followed by restriction enzyme digestion and fragment length polymorphism (RFLP) by agarose gel electrophoresis. A simplified protocol of gDNA preparation for PCR from the hair and semen of pig was established. The template DNA used in PCR was released from the hair roots or sperm by proteinase K digestion. Heat rather than phenol/chloroform extraction was used t inactivate proteinase K. The resultant digested mixture was then ready for PCR analysis. Identical genotypes were obtained from this crude prepared DNA as well as the pure DNA extracted from blood of the same animal by PCR and the following RFLP genotyping. |
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