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題 名 | Glycine Formosana Hosokawa in Taiwan: Pod Morphology, Allozyme, and DNA Polymorphism=臺灣野生大豆Glycine formosana種子形態、同功異構酶及DNA多形性之研究 |
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作 者 | 曾富生; 蔡憲宗; 阿部純; 吳詩都; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 40:4 1999.10[民88.10] |
頁 次 | 頁251-257 |
分類號 | 434.214 |
關鍵詞 | 莢果; 同功酶; DNA多形性; Allozyme; DNA polymorphism; Glycine formosana; G. soja; Pod; Seed; |
語 文 | 英文(English) |
中文摘要 | Glycinformosana Hosokawa分布於台灣桃園縣關西、橫山、大溪一帶之路旁及河旁之灌木草原地,為一攀緣纏繞性強之一年生植物。過去一直被認為與中國及日本的 G. soja同種。最近,1992 年 Tateishi and Ohashi 根據植物形態,因與 G.soja呈連續變異,而認為是地理上的亞種稱為 G.max subsp. formosana (Hosokawa)Tateishi et Ohashi。本試驗為瞭解這種野生大豆與中國、韓國及日本之 G. soja 的差異,將材料種於中興大學,進行莢果、同功異構?及 DNA 多形性之探討。 Glycine formosana 之種子較小,與中國、韓國及日本的 G. soja有顯著差異。9種同功異構?16基因座中,一個基因座 Lap1-d 僅存於 G. formosana。以 25 條引子對所有的樣本進行 RAPD 分析 , 有 21 條引子產生 DNA 放大產物 , 其中 14 條 引子之產物具有多形性。共產生 132 條 DNA 的放大產物,有 84(64%) 條放大產物具多形性。依條帶之出現計算各樣品間之相似度得:G. formosana 種內為 0.885~0.887, 幾乎無遺傳變異,而與中國及韓國有差異,但與日本的 G. soja間較類似。 |
英文摘要 | Glycine formosana Hosokawa is distributed over the grassland along the riverside and roadside at Dahshi, Guanshi, and Herngshan in Taoyuan county, Taiwan. It is a twining annual herb. It was long considered to be of the same species as G. sofa, distributed widely in East Asia. Because the plant showed a continuous variation with G. sofa in appearance, Tateishi and Ohashi (1992) considered it a geographic subspecies and renamed it G. max subsp. formosana (Hosokawa) Tateishi et Ohashi. This study attempts to determine the differences between G. formosana and the G. sofa collected in China, Korea, and Japan. The materials were planted at National Chung-Hsing University. The pods, allozymes, and DNA polymorphisms were investigated. Glycine form osana has small seeds which are significantly different from the G. sofa in China, Korea, and Japan. Lapl-d - one of the 16 loci of 9 allozymes exists only in G. formosana. Twenty-five random sequence 10-mer primers were employed in RAPD analysis for all samples. Twenty-one produced bands, and 14 of those showed polymorphisms. One hundred and thirty-two bands were produced, and 84 bands (64%) showed polymorphisms. Based on the appearances of markers, the genetic similarity coefficients were calculated. Among different samples of G. formosana, few genetic variations were observed (0.8850.887). However, G. formosana showed marked differences from G. sofa in China and Korea and nested within the Japan accessions. |
本系統中英文摘要資訊取自各篇刊載內容。