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題名 | 串聯電極式壓電石英晶體感測器之多通道系統於大腸桿菌的總生菌數檢測=Determination of Total Population of E. coli Based on a Multi-Channel Series Piezoelectric Quartz Crystal System |
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作者 | 張炳華; 張珍琦; 曾泰源; 張憲彰; | 書刊名 | 中華醫學工程學刊 |
卷期 | 19:3 1999.09[民88.09] |
頁次 | 頁209-217 |
分類號 | 410.1644 |
關鍵詞 | 多通道串聯電極式壓電石英晶體; 電導度法; 大腸桿菌; 頻率檢測時間; 總生菌數; 抗生素; Multi-channels series piezoelectric quartz crystal; Conductimetry; Escherichia coli; Frequency detection time; Total viable count; Antibiotic; |
語文 | 中文(Chinese) |
中文摘要 | 多通道串聯電極式壓電石英晶體感測系統( Multiple Channels for Series Piezoelectric Quartz Crystal, MSPQC ),運用高頻多工器與分時多工技術增進串聯式壓 電石英晶體感測器( Series Piezoelectric Quartz Crystal, SPQC )之檢測能力, 使之 具有同時能檢測多重樣品之功能。非但仍保有習用 SPQC 系統之特性與對微量電導變化的高 靈敏性, 而且系統可達 1nS/Hz 之解析度與 10 Hz/day 之系統穩定度。 本研究自製出了 MSPQC 量測系統,並提出一快速而準確方法,以對大腸桿菌( Escherichia coli )之總生 菌數做即時性之量化分析。 結果顯示其檢測能力為 10 ∼ 10 �� cells/ml,且檢測時間為 8 至 1.5 小時之間呈相對應之關係。 經比較 MSPQC 系統與直接電導度法之靈敏度, 若以 10 �� cells/ml 之 E. coli 為例, 前者之檢測時間較後者約快 12.6%。 最後我們亦將 MSPQC 系統應用於大腸桿菌對抗生素抗藥性研究上,結果由頻率響應之經時行為顯示出,本 系統能量化出藥物濃度對細菌生長代謝抑制能力的影響性,因此對間接式電導度分析於生物 性檢體的快速檢測,可提供另一簡便而又可靠之分析工具。 |
英文摘要 | Piezoelectric quartz crystal (PQC) with a pair of series electrodes was revised through a high frequency switcher and a time-division multiplexing technique to form a multi-channels series piezoelectric quartz crystal (MSPQC system). This analytical system can output the distinctive frequency based on the conductance change for individual working cell. The characteristics of MSPQC system were evaluated as same as the single channel PQC, e.g., the each channel sensitivity for conductance approximated to nS/Hz, and the long-term stability was limited in 10 Hz/day. In this study, a home made system of MSPQC was proposed as an accurately and rapidly quantitative tool for determining the Escherichia coli (E. coli) population. The sampling range of bacteria was 10 ∼ 10 �� cells/ml and the detection time of frequency (FDT) responded to former was 8 ∼ 1.5 hrs. The MSPQC response for total viable count of E. coli was compared with direct conductimetry under a consistent condition simultaneously, the experimental result showed that the FDT was faster than the detection time of conductimetry as 12.6%. The inhibited capability of antibiotic was also estimated with MSPQC system, and the concentration effect on E. coli showed a significant difference for cells metabolism. However, this method supplied an alternative idea for simple and reliable conductimetry, and the analytical efficiency of MSPQC system for microorganism was obviously enhanced than the used manner. |
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