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題 名 | 早期失聰之大白鼠經耳蝸電極植入刺激後其聽性腦幹之變化=Changes of the Auditory Brainstem in Early Deafened Rats After Cochlea Electric Stimulation |
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作 者 | 廖文輝; | 書刊名 | 中華民國耳鼻喉科醫學雜誌 |
卷 期 | 34:5 民88.09-10 |
頁 次 | 頁373-378 |
分類號 | 416.865 |
關鍵詞 | 聽覺傳入神經阻斷; 人工電極植入; NMDA受體第壹次成份; Auditory deafferentation; Cochlear implant; NR1 subunit; |
語 文 | 中文(Chinese) |
中文摘要 | 背景:利用早期聽覺傳入神經阻斷後的大白鼠,進行自製單極人工電極植入手術 及通電刺激後,比較具聽覺腦幹神經元的變化情形。 方法:大白鼠於出生後第7天起,每天皮下注射amikacin 5OOmg/kg,連續10天,於第30天 以聽性腦幹反應測試證實已為聽覺傳入神經阻斷,計有34隻。並於第40天起,施行自製單 極人工電極植入手術,而於第45天起進行每天2小時的通電刺激共15天,此為人工電極植 入組,成功通過電極聽性腦幹反應測試計有4隻。收集這4隻與聽覺神經阻斷組(5隻)和正 常對照組(5隻);皆於出生後第60天,取下其腦幹組織標本;經聽覺腦幹冷凍切片後,一齊 進行NR-1次成份的原位雜交法分析比較。 結果:早期聽覺傳入神經阻斷,可以造成耳蝸核及下丘部位,神經元細胞的面積減少分別 為65.7%及4.7%(具統計上差異),而其NMDA receptor 1 subunit(NR1)次成份表現呈減少 的情形。人工電極植入後可以使其面積分別增加2.49%及1.6%(不具統計上差異),且其 NR1次成份的表現也呈增加的情形。 結論:人工電極植入及刺激對於聽覺神經元細胞的存活維持應有幫助;同時可使得大白鼠的 聽覺腦幹由的NR1次成份其表現呈增加的情形;可能意味著其對於神經元的細胞密度及面積 的維持,有著相互的關連。 |
英文摘要 | Background: We followed our previous animal model of early auditory deaf-ferentation in rats. In this study, we tried to find the effect of cochlear implant stimulation on the auditory brainstem. Methods: In the auditory deafferentation group (34 rats) complete hearing loss, defined by an absence of brainstem evoked responses to click stimulation at 110 peak SPL, was induced by subcutaneous injections of amikacin at 500 mg/kg body weight from the 7th to 16th post-natal day (P7 to P16). In the cochlear implant group we implanted a single channel homemade cochlear prosthesis on P40 in some of the auditory deafferentation group (5 rats). We gave electric cochlear stimulation from P45 to P60. After ensuring that the prosthesis worked evenly by electrically induced auditory brainstem response on P45, due to failure of implant and death, we 1Qft 4 rats to continue the study. All rats in these two groups and also in the normal control group (5 rats) were sacrificed and decapitated on P60. We collected the brainstems of rats, cut them into sections in a cryostat at -20 'C , and performed an in situ hybridization study of NR1. Result: The neuron surface of the cochlear nucleus (CN) and inferior colliculus (IC) decreased 65.7% and 4.7% respectively after early auditory deaf-ferentation. The expression of NR1 subunit decreased. After cochlear implant stimulation, the neuron surface of the CN and IC, 2.49% and 1.6% respectively, Although this was not statistically significant. There was a significant increase in the expression of NR1. Conclusion: A cochlear implant can play an important role in maintaining of auditory brain stem neurons. The increased expression of NR1 subunit may have some relationship with maintenance of neuron survival. |
本系統中英文摘要資訊取自各篇刊載內容。