頁籤選單縮合
題 名 | 以毛細管電泳法檢驗飼料中Clenbuterol、Salbutamol及Terbutaline之殘留=Capillary Electrophoresis for the Confirmatory Assay of Clenbuterol, Salbutamol and Terbutaline in Feeds |
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作 者 | 戴東發; 華宏達; 潘憶文; 尤俊傑; 劉朝鑫; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 25:3 1999.09[民88.09] |
頁 次 | 頁183-190 |
分類號 | 437.111 |
關鍵詞 | 飼料; 藥物殘留; β-agonists; ELISA; Capillary electrophesis; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究旨在以簡單、快速之方法同時檢測飼料中添加之Clenbuterol、Salbutamol及Terbutaline。本檢驗法先將飼料樣品以0.01 N HCl簡單之萃取後,萃取液再以檢測β-Agonists之酵素免疫檢測套組(ELISA kit)作篩選檢驗;陽性樣品以C18固相萃取管萃取後,萃取液經75μm矽膠毛細管,於pH8.8磷酸緩衝液,15KV,23℃,UV 200 nm條件下之毛細管電泳(CE)進行定性和定量之確認分析。以ELISA kit檢測飼料中上述三種β-Agonists化合物,其最低檢測感度皆為0.1 ppm。CE之定量檢測三者之最低確認檢測感度皆為0.5ppm;此檢驗法之回收率對Clenbuterol為63-67%,Salbutamol為88-95%,Terbutaline為55-56%。本研究顯示於含0.1 ppm以上上述三種藥物之飼料皆可同時快速、可靠、準確之檢測。 |
英文摘要 | This paper presents a simple method for the simutaneous determination of β-Agonists like Salbutamol, Clenbuterol and Terbutaline from feeds. These were extracted simply by adding 0.01 N HCl to the sample, and followed by Enzyme-linked lmmunosorbent Assay (ELISA). The positive sample was extracted using C�� solid-phase technique. After extraction, the eluent is injected to the Capillary Electrophoresis (CE) system with phosphate buffer pH8.8, (15KV, 23℃,UV 200 nm). The detection limits for these compounds are 0.1 ppm by ELISA for screen test, and 0.5 ppm by CE for confirmatory assay. The recovery rates for Salbutamol, Clenbuterol and Terbutaline are 88-95%, 63-67% and 55-65% respectively. Our results show that this is a rapid、reliable and accurate method for the determination of Salbutamol, Clenbuterol and Terbutaline simutaneously in feeds with a detection limit of 0.1 ppm. |
本系統中英文摘要資訊取自各篇刊載內容。