頁籤選單縮合
題名 | Separation of Cholesterol Esterase in Cloud Point Extraction System=利用濁點萃取進行膽固醇脂解酶分離之研究 |
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作者姓名(中文) | 江禎立; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 30:2 1999.03[民88.03] |
頁次 | 頁171-176 |
分類號 | 460.02 |
關鍵詞 | 濁點萃取; 膽固醇脂解酶分離; Cloud point extraction; Cholesterol esterase; Partition coefficient; |
語文 | 英文(English) |
中文摘要 | 本文嘗試利用非離子型界面活性劑進行膽固醇脂解�t液--液兩相濁點萃取。為了 找尋最適當操作條件,我們分別探討不同聚氧乙烯界面活性劑( C �� E �N,C �� E �N,C �� E �腹^、界面活性劑組成與含量、 磷酸鹽濃度、溫度、二級操作對濁點溫度與酵素分離 的影嚮。 所得最適當的操作條件為:以 1 %( w ╱ v ) 5 Lauryl Ether ( C �� E �N )在 22.8 ℃下進行,經過二級操作,可得到約 5 倍濃縮濃度與 77 %回收產率。 |
英文摘要 | The use of cloud point extraction system with nonionic polyoxyethylene surfactants (C �� E �N, C �� E �N, and C �� E �� ) was carried out for direct solubilization and extraction of membranebound cholesterol esterase (cholesterol ester hydrolase; CEH) from Pseudomonas fl. The effects of various operational parameters (surfactant weight percentage, temperature, phosphate concentration and composition of surfactant mixture) on the CEH partition behavior have been investigated. The partition coefficient and yield changed insignificantly with increasing surfactant (w/v) percentage for both C �� E �N and C �� E ��. The cloud point also shows a linear dependence on weight percentage of the shorter headgroup surfactant. The dependence is described approximately as T □= 20.75-0.309 W and T □= 32.82-0.243 W, for C �� E �� /C �� E �N and C �� E �N /C �� E ��, respectively. After a two-stage separation, an initial 5-fold concentration with 77% yield has been achieved under the condition of 1% (w/v) of C �� E �N at 22.8 ℃ without potassium phosphate addition. |
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