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題名 | 以固相萃取法及石墨爐式原子吸收光譜儀分析豬肉中血質鐵之含量=A New Method for Determining Heme Iron in Pork using Solid Phase Extraction and Graphite Furnace Atomic Absorption Spectrophotometer |
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作者 | 陳石松; 張為憲; 周薰修; | 書刊名 | 藥物食品分析 |
卷期 | 6:2 1998.06[民87.06] |
頁次 | 頁529-536 |
分類號 | 341.91 |
關鍵詞 | 固相萃取法; 石墨爐式原子吸收光譜儀; 豬肉; 血質鐵; Solid phase extraction; Graphite furnace atomic absorption spectrophotometer; GFAAS; Pork; Heme iron; HFe; |
語文 | 中文(Chinese) |
中文摘要 | 本研究探討以固相萃取法及石墨爐式原子吸收光譜儀測定豬肉中血質鐵之含量。 其方法為將豬肉以80%酸性丙酮溶液(pH<1)均質萃取,取上澄液10 ml加水10 ml,通 過碳18處理匣,血質可被吸附,再以80%丙酮溶液5 ml沖提,將沖提液以偏極化茲曼石墨 爐式原子吸收光譜儀直接測定其吸光度,由標準曲線計算其含量。添加含血質鐵1.75、2.50 及3.50μg/g之肌紅素於豬肉中,回收率為97.9、98.1及99.2%;添加含血質鐵3.00、7.00 及10.00μg/g之血紅素於豬肉中,回收率為98.1、98.8及99.3%。 |
英文摘要 | A new method of selective determination of heme iron in pork was developed. First heme iron in pork was extracted with 80% acetone acidified with 2% hydrochloric acid to below pH 1.0. After adjusting the concentration of acetone to 40%, the solution was passed through a Sep-Pak C18 cartridge. Heme was adsorbed on the cartridge, and was then eluted with 80% acetone. The eluent was analyzed for heme iron with a graphite furnace atomic absorption spectrophotometer. Recovery studies were performed by adding 1.75, 2.50 and 3.75 μg HFe/g myoglobin and 3.00, 7.00 and 10.00μg HFe/g hemoglobin into the pork. Recoveries were in the range of 97.9~99.3%. 9.3%. |
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