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題 名 | Characterization of Zucchini Yellow Mosaic Virus (ZYMV) Isolates Collected from Taiwan by Host Reactions, Serology, and RT-PCR=以寄主反應、血清學及反轉錄酶連鎖反應分析臺灣矮南瓜黃化嵌紋病毒之特性 |
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作 者 | 林詩舜; 侯豐男; 黃秋雄; 葉錫東; | 書刊名 | 植物保護學會會刊 |
卷 期 | 40:2 1998.06[民87.06] |
頁 次 | 頁163-176 |
分類號 | 373.9 |
關鍵詞 | 矮南瓜黃化嵌紋病毒; 寄主反應; 免疫血清擴散反應; 反轉錄酶連鎖反應; 限制酶; Zucchini yellow mosaic virus; Host reaction; Immunodiffusion; RT-PCR; Restriction enzyme; |
語 文 | 英文(English) |
中文摘要 | 1998 以寄主反應、血清學及反轉錄酵素連鎖反應分析臺灣矮南瓜黃化嵌紋病毒之特性 植保會刊 00:000-000. (1國立中興大學農業生物科技學研究所;2臺灣省農業試驗所植物病理系;3國立中興大學植物病理學系) 本研究以寄主反應、免疫擴散反應及反轉錄酵素連鎖反應 (RT-PCR) 產物的限制酵素多型性分析來比較從臺灣不同瓜類產區收集之矮南瓜黃化嵌紋病毒 (zucchini yellow mosaic virus, ZYMV) 分離株之異同。所得三十三個病毒分離株在矮南瓜葉片上所造成的病徵可歸納成三類:黃化嵌紋與扭曲窄化、黃化嵌紋與扭曲、黃化嵌紋與脈綠。除了 ZYMV-CY2 分離株在絲瓜及胡瓜上產生壞疽病徵之外,其它分離株在甜瓜、絲瓜及胡瓜上則產生黃化嵌紋病徵。以臺南 ZYMV-TN3 分離株純化所得之鞘蛋白所製備的抗血清,經免疫血清擴散反應證實其與其他分離株在血清反應上是不可區分的。為了提供更快更靈敏的診斷工具,反轉錄酵素連鎖反應之診斷方法被發展出來,以針對 ZYMV NIb 及 CP 基因所設計的專一性核酸引子經由 RT-PCR 對三十三個分離株均可增幅出一個 0.76 kb 的 DNA 片段。為了更進一步分析具代表性的五個 ZYMV 分離株 CP 基因的變化,利用 TagI、EcoRV 及 NlaIII 剪切這些 CP 基因 N 端變化區的 RT-PCR 產物以比較其異同。結果顯示 ZYMV-TC1、CY2 及 TNML1 的親緣性較接近,而 ZYMV-TN3 及 NT1 與上述三分離株親緣關係比較疏遠。 |
英文摘要 | Zucchini yellow mosaic virus (ZYMV) isolates collected from different areas of Taiwan were characterized by host reactions, sodium dodecyl sulfate (SDS) immunodiffusion tests, and restriction enzyme polymorphism of reverse transcription-polymerase chain reaction (RT-PCR) products. The sympotoms on zucchini squash by the 33 ZYMV isolates were divided into three types: yellow mosaic with leaf distortion and narrowing, yellow mosaic with leaf distortion, and yellow mosaic with vein-banding. There ZYMV isolates induced yellow mosaic sympotoms on melon, sponge gourd and cucumber, except that the isolate CY2 acused necrosis on the leaves of sponge gourd and cucumber. In SDS-immunofiffusion tests, the antiserum prepared against the isolate TN3 reacted with all the 33 ZYMV isolates indistinguishabaly by formation of homogenous precipition lines without spurs. In order to provide a fast and sensitive diagnostic tool, a RT-PCR detection method was developed. A 0.76 kb DNA fragment was amplified from the 33 isolates by RT-PCR using primers specific to the NIb and CP genes of ZYMV. To further analyze the variability of the CP genes of five selected ZYMV isolates, EcoRV, TaqI, and NlaIII digestion patterns of RT-PCR products, which were amplified from the variable N-terminal halves of the CP genes, were compared. The results showed that the isolates TC1, CY2, and TNML1 are closely related, while TN3 and NT1 are more variable. |
本系統中英文摘要資訊取自各篇刊載內容。