查詢結果分析
相關文獻
- Leaf and Stem Crown Galls on Perennial Asters Caused by Agrobacterium Tumefaciens in Taiwan
- 下頜腺腫瘤--本土病例低惡性率之臨床意義
- Germline RET Proto-Oncogene Mutations in Two Taiwanese Families with Multiple Endocrine Neoplasia Type 2A
- Correlation between Urinary and Serum Prostate--Specific Antigen
- Renal Cell Carcinoma Presented as a Traumatic Ruptured Kidney
- 惡性肺癌轉移至牙周組織之病例報告
- A Patient of Primary Hyperparathyroidism with Full-Blown Bone Changes Simulating Malignancy
- Concomitant Measurement of CA 125 and CA19-9 in Detecting Ovarian Malignancy: A Review Study of 64 Cases
- 二氧化碳顯影電腦斷層與二氧化碳顯影超音波於肝臟腫瘤之比較
- Primary Malignant Fibrous Histiocytoma of Lung: A Case Report
頁籤選單縮合
題名 | Leaf and Stem Crown Galls on Perennial Asters Caused by Agrobacterium Tumefaciens in Taiwan=臺灣地區宿根紫苑莖部及葉片腫瘤是由細菌性癌腫病菌(Agrobacterium tumefaciens)所引起 |
---|---|
作 者 | 陳福旗; 許秀惠; 洪秀桃; 陳明昭; 林俊義; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 40:3 1999.07[民88.07] |
頁次 | 頁237-242 |
分類號 | 373.9 |
關鍵詞 | 癌腫病菌; 紫苑屬; 生物型1; 腫瘤; 選擇性培養基; Agrobacterium tumefaciens; Aster sp.; Biovar 1; Crown gall; Selective medium; |
語文 | 英文(English) |
中文摘要 | 宿根紫苑為一新興切花作物, 有多種花色,其中兩個品種'紫孔雀' , 及‘白孔雀' ,在 85 年春於屏東地 區發現在修剪過的植株基部、根冠、葉片及切口處均長出大小不等的腫瘤 , 大者約可達直徑 5 公分 , 有 些植株受昆蟲咬食後之葉片會延著傷口長出小形腫瘤。不同品種間發生腫瘤的比率有差異, 紫孔雀品種在田間約有 90% 以上的植株都發現產生腫瘤 , 白孔雀之發生率則較低, 約 25%, 最近又觀察到另一個新引 入的白花品種部份植株也在莖部及葉片傷口產生較小腫瘤。紫孔雀腫瘤利用無菌水萃取並以 NASA 培養基分離得到磚紅色之圓形菌落, 以 YM 培養基純化之菌落為白色、突起、光滑且為圓形。經以 BiologGN Microplate (Biolog, Inc., Hayward,CA,USA) 培養鑑定為 Agyobacteriumtum tumefaciens, 當以生化檢定方法與標準 biovar。當以生化檢定方法與準biovar 1菌系與Chry5 比較時 , 顯示多數紫苑菌系均屬於同一biovar 。將細菌以 YM 或液體之 YM 培養 基培養過夜後 , 以針刺或解剖刀接種於落地生根葉片、農友 301 番茄及煙草 (Nicotiana tabacum cv. Xanthi NC) 幼苗之葉片或莖部 ( 番茄及煙草 ), 番茄及煙草在接種後 6~8 天於接種處產生白色腫瘤,其寬度約1~15公分,而在落地生根葉片上之腫瘤約在 8~10 天才開始形成且較小 ,且呈柱狀或不規則圓球狀。將 分離之菌落培養成懸浮液並以剪刀回接紫苑葉片,在lO~12天後,於切口位置長出小瘤,對照組則沒有變化。由以上的試驗結果 , 證實造成'紫孔雀',腫瘤病之病原茵為農桿癌腫病菌 Agrobacterium tumefaciens biovar 1。 |
英文摘要 | Leaf, stem, and crown galls induced by Agrobacterium tumefaciens were observed on field grown perennial asters (Aster spp.). Plants of purple flowers were more susceptible to infection than white flowers. The occurrence ranged from 25% in white flowered and 90% in purple flowered plants. Galls also occurred on leaves wounded by insect bites or mechanical shearing. Agrobacterium tumefaciens was isolated from crown and leaf galls with a selective medium NASA. The bacterial isolate was identified as A. tumefaciens using the Biolog GN system. Inoculation of selected A. tumefaciens strains on Kalanchoe pinnata leaves resulted in gall formation 8-10 days afterward. Several other A. tumefaciens strains from different gall samples also caused gall formation 6-8 days after inoculating on the stems of tobacco, and tomato seedlings. Re-inoculating virulent strains by scissors onto healthy aster leaves also induced galls 10 to 12 days after cut-inoculation. Biochemical tests of most Agrobacterium strains from aster galls showed that they belong to biovar 1. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。