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題名 | 三種SOD活性測定法之比較=Comparison of Three Methods for SOD Activity Determination |
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作者 | 黃政弘; 陳惠婷; 凃瑞澤; Huang, Jeng-hong; Chen, Hui-ting; Too, Jui-rze; |
期刊 | 大葉學報 |
出版日期 | 19990600 |
卷期 | 8:1 1999.06[民88.06] |
頁次 | 頁101-109 |
分類號 | 463 |
語文 | chi |
關鍵詞 | 超氧歧化酶; 黃嘌呤; 黃嘌呤氧酶; NBT光還原反應; Pyrogallol自氧化; Superoxide dismutase; SOD; Xanthine; Xanthine oxidase; Photo-reduction of NBT; Autoxidation of pyrogallol; |
中文摘要 | 超氧自由基是氧化還原的中間產物,具毒性,能造成細胞受損。而超氧歧化�@(SOD)則有清除超氧自由基的功能。本研究的目的是探討三種SOD的活性測定法,並比較其優劣。在黃嘌呤+黃嘌呤氧化�@法的反應中,黃嘌呤氧化�@的含量為0.05 U時,對於SOD的感受性最高。但SOD的活性小於0.5 U時,則無法正確測得其活性值。反應溶液在配製後經過24 hr,即不再適用於SOD的活性測定。NBT的光還原反應對SOD的感受性比黃嘌呤氧化�@系來的高,此法對於SOD在0.0625-5.0 U的範圍內有良好的靈敏度,但反應混合液超過四天便不能再使用。Pyrogallol的自氧化法,對於SOD在0.0625-5.0 U的範圍內有良好的感受性。對於SOD的靈敏度,pyrogallol自氧化反應與NBT光還原法不相上下,且pyrogallol於0.2 M的HCl酸性溶液中可保存較長時間,對於冗長的純化過程,是一種很適合的活性測定法。 |
英文摘要 | Superoxide radicals are intermediates in many redox reactions occurring in living cells. These radicals are toxic and can cause cell damage. The superoxide dismutase (SOD) can scavenge superoxide radicals. The purpose of this study is to examine three methods for determining SOD activity and to compare their merits and storage stability. The xanthine oxidase method shows that the bovine SOD is most sensitive to a 0.05 U content of xanthine oxidase. When the SOD activity is less than 0.5 U, a precise amount of SOD cannot be measured from the changes of absorbance. The xanthine oxidase solution becomes ineffective for determining SOD activity after 24 hours of storage at room temperature. The NBT method more effectively determines the SOD activity than the xanthine - xanthine oxidase method. Experimental results reveal that the NBT method is suitable to determine the presence of bovine SOD in the range of 0.0625 and 5.0 U. The NBT solution becomes ineffective in determining SOD activity after 4 days of storage at room temperature. Experimental results reveal that pyrogallol autoxidation method is very effective to determine bovine SOD activity if its range is between 0.0625 and 5.0 U. This is the same range for the NBT photo-reduction method. In addition, the pyrogallol solution is still stable for two weeks of storage at room temperature. Comparison of these methods reveals that the pyrogallol autoxidation method is most convenient for determining SOD activity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。