查詢結果分析
相關文獻
- 以銀合歡種子內含成份Mimosine輔助提高化學治癌效果之研究
- 銀合歡種子內含成份Mimosine在活體動物抗癌效果之研究
- P53抑癌基因與婦科腫瘤
- 抑癌基因在白血病發生發展中作用
- FHIT (fragile histidine triad)基因在子宮頸癌前期病變之分析
- P53抑癌基因在卵巢透亮細胞癌中不常發生
- Correlation of Elevated mRNA Expression of p53 Gene and Repair Genes with Lung Cancer Chemoresistance
- 抗癌藥物Mimosine衍生物的合成及其抗癌效果之研究
- Two-hit Hypothesis of Tumor Suppressor Gene and Revisions
- 以臺灣產茄科中藥調控肝細胞腫瘤中p53及Rb抑癌基因表現與調節修補DNA變異之酵素活性及其在抗腫瘤機轉上的研究
頁籤選單縮合
| 題 名 | 以銀合歡種子內含成份Mimosine輔助提高化學治癌效果之研究=Study of Enhancement of Sensitivity of Hepatoma and Lung Cancer Cells to Chemotherapeutc Drugs by Mimosine from Seeds of Leucaena Benth |
|---|---|
| 作 者 | 洪文俊; | 書刊名 | 中醫藥年報 |
| 卷 期 | 17:3 1999.05[民88.05] |
| 頁 次 | 頁555-598 |
| 分類號 | 418.5 |
| 關鍵詞 | 抑癌基因; 週期素; 化學治癌藥物; Mimosine; Tumor suppressor genes; Cyclins; p21WAF1; p27KIP1; Chernotherapeutic drugs; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 細胞癌化的過程中,p53抑癌基因的變異非常常見,此基因的缺 損和癌細胞對化學治癌藥物的抗藥性有著密不可分的關係。許多化 學治癌藥物會增加癌細胞p53基因的表現,進而誘導p21WAF1基因 的表現來抑制癌細胞的增生並且引發癌細胞自然死亡(Apoptosis)。許 多癌細胞其p53基因的有功能缺損,所以添加化學治癌孳物並無法有效 的促進p53與WAF1基因的表現,也無法有效的抑制癌細胞的生長。 近來的研究顯示mimosine,一種由銀合歡種子萃取出的植物特有的 氨基酸,在很低濃度時便可以在乳癌細胞內不經由p53直接活化 p21WAF1基因的表現。本研究的目的在探討此氨基酸對國人最常罹 患的肝癌及肺癌,是否也可以促進p21WAF1的表現,我們進一步探 討此氨基酸是否能提高化學治癌藥物的療效,以評估它的臨床實用 性。我們藉由測定NDA合成,細胞生長曲線、細胞週期分布及洋菜 瓊膠群落生成來分析mimosine對肝癌及肺癌細胞生長的影響。我們 以北方及西方點墨法來探討此氨基酸對細胞週期調控蛋白,包括週 期素及週期素依賴性蛋白激脢抑制蛋白表現的影響,並利用蛋白激 脢活性分析來偵測週期素與蛋白激脢複合體功的改變。最後,藉由 測定細胞存活情形來評估mimosine配合臨床上使用的化學治癌藥物 是否可加強其抗癌效果。我們的實驗結果發現mimosine可有效抑制 肝癌與肺癌細胞的增生,此藥物的作用主要是阻斷細胞由G1期進入 S期,它也可明顯的抑制癌細胞的腫瘤生成能力減少癌細胞在洋菜瓊 膠形成的群落數目。Mimosine是藉由不同的作用機轉來抑制癌細胞 生長,它可減少癌細胞週期素D1的合成也可增加抑制蛋白(p21WAF1 及p27KIP1)的含量使癌細胞無法進入S期。我們也發現mimosine與化 學治癌藥物對癌細胞的毒殺作用具有加成性,它是藉由引發癌細胞 的凋亡來消滅癌細胞。綜合研究結果,我們結論mimosine有強烈的 抗癌效果而且可配合化學治癌藥物使用以提高療效。進一步的活體 動物實驗,可評估該氨基酸在活體內的抗癌效果及未來臨床的應用 性。 |
| 英文摘要 | Mutation of p53 tumor suppressor gene are frequently found in the process of carcinogenesis. Recent results also suggest a slose relationship between the defect of p53 gene and the development of drug-resistance in tumor cells. many anti-cancer durgs enhance p53 gene expression and then activate subsequent p21WAF1 gene expression. The p21WAF1 protein may inhibit cell grough and induce apoptosis in cancer cells. However, chemotherapeutic drugs could not increase the p53 and p21WAF1 gene expression in p53 defect cancer cells and could not inhibit demonstrated that mimosine, a plant specific amino acid extracted from the seeds of Leucaena Glauca Benth, could activate p21WAF1 gene expression independent of p53 at very low concentration in breast cancer cells. The aim of our work is to study whether mimosine could enhance p21WAF1 expression in liver and lung cancers Furthermore, we also evaluate the effectiveness of the combination of mimosine and chemotherapeutic drugs in cancer treatment. We investigate the effect of mimosine on cell growth of liver and lung cancer cells by using DNA synthesis assays, cell proliferation assays, flow eytometic analysis and in vitro colony formation assays. We also examine the effect of this drug on the expression of cyclins and cyclin-dependent kinase inhibitors by Northern and Westen blotting. We use a in vitro kinase assay technique to study the change of the kinase activity of cyclin-CDK complexes. Finally, the anit-cancer effect of mimosine in combination with chemotherapeutic drugs was evaluated by MTT assays. Our results demonstrated that mimosine inhibited the proliferation of liver and lung cancer cells and blocked the progression of cell cycle from G1 to S phases in these cells. This drug reduced the formation of colony of cancer cells in soft agar. Cell growth arrest induced mimosine is mediated by multiple mechanisms in human cancer cells. Mimosine may act via inhibition of the synthesis of cyclin D1 or enhancement of the expression of p21WAF1 and p27KIP1 to block the entry of cells into S phase. There is an additive cytocidal effect of mimosine in combination with chemotherapeutic drugs. Additionally, long-term treatment of mimosine induced apoptosis in liver and lung cancer cells. We conclude that mimosine is a potent anti-cancer agent and this amino acid can enhance the cytocidal effect of chemotherapeutic drugs. Future study in nude mice will be helpful to evaluate the anti-cancer effect of mimosine in vivo. |
本系統中英文摘要資訊取自各篇刊載內容。