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題名 | Cytostructure, Lipopolysaccharides, and Cell Proteins Analysis from Rhizobium fredii=根瘤菌Rhizobium fredii細胞結構、脂多醣體及胞蛋白之分析 |
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作者 | 楊鳳玲; 林良平; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 39:4 1998.10[民87.10] |
頁次 | 頁261-267 |
分類號 | 430.16341 |
關鍵詞 | 抗細胞蛋白抗體; 抗LPS抗體; 胞蛋白; 細胞結構; 脂多醣體; 快生性根瘤菌; 大豆; Anti-cellular protein antibody; Anti-LPS antibody; Cell proteins; Cytostructure; Lipopolysaccharides; Rhizobium fredii; Soybean; |
語文 | 英文(English) |
中文摘要 | 在農業之應用上,根瘤菌和豆科植物共生時所扮演的角色已經被詳加研究。在本 文中採用數種臺灣本土分離之根瘤菌,對它們的細胞結構、胞蛋白及脂多醣體加以分析,並 與慢生性根瘤菌標準菌株做比較,它們的細胞結構特色在於有聚-β-基丁酸鹽(poly-β- hydroxybutyrate, PHB)以及聚磷酸鹽(polyphosphate)之存在。對脂多醣體及胞蛋白以 電泳及免疫方法加以分析,發現其具有菌株專一性及多樣性。 |
英文摘要 | The symbiotic roles of rhizobia with legumes have been extensively investigated for agricultural applications. We isolated several strains of rhizobia from Taiwanese soils and analyzed their cellular proteins and lipopolysaccharide (LPS) profiles as well as their ultrastructure by electron microscopy. It was found that the flagella have different forms and locations on the surface of fast-growing and slow-growing rhizobia. In addition, the results of their ultrastructural visualization confirmed that poly-B-hydroxybutyrate (PHB) and polyphosphates are present in rhizobia. The ladder profiles of LPS and cellular proteins on sodium dodecylsulfate- polyacrylamide gel electrophoresis (SDS-PAGE) indicated that different strains of rhizobia possess different profiles, but strains which belong to the same species have similar profiles. It was also shown by Western blot and immunoassay that strian specificity exists between rhizobia cellular proteins and the antisera of anti-rhizobia cells. These results confirm that indigenous R.fredii exhibits a significant difference from other species of Rhizobiaceae. |
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