頁籤選單縮合
題名 | Chemical Characterization of α Hormones of Phytophthora Parasitica=Phytophthora parasitica之α荷爾蒙之化學特性 |
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作者 | 陳麗鈴; 唐崇實; 柯文雄; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 40:1 1999.01[民88.01] |
頁次 | 頁79-85 |
分類號 | 435.17 |
關鍵詞 | α荷爾蒙; 真菌有性生殖; Phytophthora parasitica; α hormones; Sexual reproduction of fungi; |
語文 | 英文(English) |
中文摘要 | 本文敘述提取、分離、以及化學分析植物疫病菌Phytophthora parasitica 所產生之性荷爾蒙。將A1配對型或A2配對型之試驗真菌分別培養於V8瓊脂 上,以有機溶劑萃取培養基,可得到α1或α2荷爾蒙之粗抽液。本實驗共使用 240公升有機溶劑萃取了40公升培養基,得到之荷爾蒙,經過酸鹼淬取分離法 測定屬於中性化合物。α1和α2不會被丙酮或毛地黃皂□(digitonin)所沈澱,也 不在皂化過程中被破壞。二者在氮氣中均能耐高溫。以氨丙基(aminopropyl)管柱 分離後其活性存在於中性脂部份。此二荷爾蒙之分子極性與單酸甘油脂類似,但 α2大於α1。α1之活性不為脂化反應所改變,但α2之活性在脂化反應後喪失, 而在鹼水解α2脂化物後其活性又可恢復。α1以半製備型之氣相層析法(GC)分 離純化在330到280℃中被洗出,但是GC配備的熱導性探測器(TCD)及火焰離子化 探測器(FID)均未偵測到吸收峰。α2不能在同樣GC條件下被氣化和收集到活性。 以上實驗結果證明P. parasitica之瓊脂培養物中含有濃度極低但活性極高之α賀 爾蒙。必須更大規模的培養才能生產出足供化學結構鑑定之量。本研究顯示α荷爾 蒙不屬於卵磷脂、醣脂、醣化物或類固醇等化合物,它們應是具氫氧基、熱安定性 之中性脂質。只有以α1具有揮發性。α2之氫氧基對其荷爾蒙活性有必要性。 |
英文摘要 | A method for the large scale hormone al andα2 extraction from agar cultures of Al and A2 mating types of Phytophthora parasitica has been developed. Using a total of 240 L of organic solvents to extract 40 L of the agar medium, sufficient amounts ofα hormones were obtained to permit characterization of their physical and chemical properties. Activities of both hormones were in the neutral fractions in acid-base partition. These hormones were not precipitated by cold acetone or digitonin, nor were their activities destroyed by saponification. Fractionation on an aminopropyl column showed that both hormones were neutral lipids with polarity similar to that of monoglycerides and thatα2 had a polarity greater than that of cil. Both hormones were stable at 200℃ under nitrogen. andαl was more volatile than α2. After acetylation, the hormonal activity ofαl was retained, while that ofα2 was greatly reduced. Base hydrolysis of the acetylatedα2 led to the restoration of its hormonal activity. Using semi-preparative gas chromatography, αl activity was eluted between 230 and 278℃ whileα2 could not be eluted under the same conditions. Results from this study suggest thatα hormones are not phospholipids, glycolipids, glycerides, or steroids, but that they are probably neutral lipids with hydroxyl functional group(s). Structural elucidation ofαl andα2, however, would require an even greater scale of operation than that provided by the present experiment. |
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