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題名 | Cytochemistry and Ultrastructure of Canine Myocardium Undergoing Global Ischemia and Reperfusion Injury=實驗犬之全心缺血再灌流損害之心肌細胞化學及超微結構之分析 |
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作者 | 袁師敏; Yuan, Shi-min; |
期刊 | The Kaohsiung Journal of Medical Sciences |
出版日期 | 19990100 |
卷期 | 15:1 1999.01[民88.01] |
頁次 | 頁1-7 |
分類號 | 415.121 |
語文 | eng |
關鍵詞 | 實驗犬; 全心缺血再灌流損害; 心肌細胞化學; 超微結構; Cytochemistry; Mitochondria; Myocardium; |
中文摘要 | 為闡明全心心肌缺血後再灌流損害之嚴重度,對實驗犬心肌之糖元及琥珀酸去氫堃(SDH)之細胞化學及蘇木素一伊紅染色、心肌細胞之超微結構進行分析。本研究選擇7只實驗犬制作全心缺血模型。分別於(1)建立體外循還環後(第一次活檢);(2)上行主動脈阻斷末(第二次活檢);和(3)去除上行主動脈阻斷鉗之後30分鐘(第三次活檢);自左心室前壁採取心肌組織標本。所有心肌標本均行細胞化學分析,後二次的心肌標本行超微結構的分析。心肌組織之平均衰壞率和心肌粒線體的表面積與體積之比率分別在電鏡檢視下和電鏡片上進行計算。蘇木素一伊紅染色,第一次活檢的心肌纖維形態正常;第二次活檢的心肌纖維呈波浪狀、部分空泡變性,核的形;第三次活檢的心肌纖維呈波狀、嚴重空泡變性。糖元染色,三次標本分別呈中等度、弱、和強陽性的反應。三次的 SDH 染色則分別呈強、和中等度的陽性反應。電鏡結果示,第二次活檢心肌Z帶嚴重扭巨,少數的粒線體參和基質呈性的浴解;第三次活檢心肌壞死區Z帶消失,心肌纖維模糊、片狀溶解,可見成簇或變形的粒線體。後二次活檢標本的心肌組織之平均衰壞率具有顯著的差異(14.88 ± 3.09%, 60.2 5± 8.55%, p=0.001)。缺血期粒線體的表面積與體積之比率較再灌流期者顯著增大(3.953 ± 1.088μm¯¹, 2.767 ± 0.933μm¯¹, p=0.041)。再灌流期心肌損害較缺血期心肌損害厲害。實驗犬心肌組織細胞化學和超微結構的變化之間有一定的關聯性。 |
英文摘要 | In order to highlight serverity of myocardial injury during the course of global ischemia and reperfusion, cytochemistry of glycogen and succinate dehydrogenase (SDH) as well as hematoxylin and eosin staining (H & E) and electron microscopy were observed in canine my cardium. Seven mongrel dogs were selected for reperfusion injury after global ischemia in this study. Myocardial biopsies were taken from the anterior wall of the left ventricle (a) after cardiopulmonary bypass (the first biopsy); (b) at the end of the aortic crossclamp (the second biopsy); and (c) 30minutes after crossclamp removal (the third biopsy). All biopsies were cytochemically assessed, and the latter two, for electron microscopic studies. The averages of myocardial necrotic rate and surface to volume ratio of myocardial mitochondria were calculated under electron microscopy and in electron microscopic slices, respectively. Myofibrillae were of normal morphology in the first biopsy; in wave-shape and partly vacuolated, with large and deformed nuclei in the second one; and in wave-shape and severely vacuolated in the third one, in H & E glycogen granules were variously stained in moderate, weak and intensive positive reactions in the three biopsies respectively in glycogen staining. SDH was stained in intensive, weak, and moderate positive reactions in three, respectively. By electron microscopy, Z bands twisted severely, and local dissolution of cristae and matrix occurred in a minority of the mitochondria in the second biopsy; and majority of the Z bands in necrotic region had disappeared, the myofibrillae were obscure and patchily dissolved. Clustered and deformed mitochondria could be found in the third biopsy. Significant difference could be noted between the averages of the second and third biopsies (14.88±3.09% vs 60.25±8.55%, p < 0.001). The surface to volume ratio of the ischemic mitochondria was much bigger than that of the reperfused (3.95±1.09μm¯¹vs. 2.77±0.93μm¯¹, p=0.041). myocardial injury was more severe in reperfusion than in ischemia myocardium. There were correlations between histobiochemical and ultrastructural alterations in damaged canine myocardium. |
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