查詢結果分析
來源資料
相關文獻
- Effect of Caloric Restriction on in Vitro Metabolism and DNA Binding of the Environmental Potent Tumorigen 7-Chlorobenz[a]Anthracene
- DNA-binding Properties of the Recombinant Nucleocapsid Protein of Classical Swine Fever Virus
- Cloning, Expressing and Purification of the Recombinant Polyomavirus VP1 and Characterization of its DNA Binding Domain
- 國王企鵝(Aptenodytes Patagonicus)性別鑑定
- The Elevation of Plasma DNA in Patients with Systemic Lupus Erythematosus Is Attributable to Increased DNA Release and Defective DNA Binding of Mononuclear Cells
- Effect of energy restriction and dietary fat on the lipid metabolism in rats
- Functional Redundancy of the Duplex Telomeric DNA-Binding Proteins in Arabidopsis
- Overexpression of Damaged-DNA-Binding Protein 2 (DDB2) Potentiates UV Resistance in Hamster V79 Cells
- 間歇性飲食的臨床相關應用
- 飼料的能量限制與蛋白質限制對於豬血液組成及生殖行為的影響
頁籤選單縮合
題 名 | Effect of Caloric Restriction on in Vitro Metabolism and DNA Binding of the Environmental Potent Tumorigen 7-Chlorobenz[a]Anthracene=環境強玫癌物7-氯苯井[a]蒽體外代謝和DNA加成物合成實驗能量限制的影響 |
---|---|
作 者 | 邱莉婷; 詹德進; 翁榮源; 符必成; | 書刊名 | 藥物食品分析 |
卷 期 | 7:1 1999.03[民88.03] |
頁 次 | 頁23-36 |
分類號 | 415.121 |
關鍵詞 | 能量限制; 7-氯苯井[a]蒽; 小鼠肝臟微粒體代謝; DNA結合; Caloric restriction; 7-chlorobenz[a]anthracene; Mouse liver microsomal metabolism; DNA binding; |
語 文 | 英文(English) |
中文摘要 | 環境污染致癌物-7-氯苯并[a]�I的代謝和DNA加成物合成實驗中,對所使用的小鼠 施予能量限制,取得 9 個月大的雄性 B6C3F �竣p鼠和 9 或 12 個月大的雌性 B6C3F �竣p 鼠之肝臟微粒體,研究其對實驗結果所造成的影響。小鼠從出生開始,以正常飲食飼養;14 週後逐步能量限制,到 16 週以餵食正常飲食之 60 %能量;9 個月和 12 個月大處理之, 製備得到微粒體。 微粒體和 7- 氯苯并 [a] �I的體外代謝實驗後,所得代謝物經過萃取、 逆相高壓液相層析儀純化後, 與標準品比對他們的紫外 - 可見光譜和質譜圖譜後,證明有 10 種代謝物,分別如下:反式 -3,4- 二氫二醇 -7- 氯苯并 [a] �I; 反式 -5,6- 二氫二 醇 -7- 氯苯并 [a] �I;反式 -8,9- 二氫二醇 -7- 氯苯并 [a] �I;反式 -10,11- 二氫二 醇 -7- 氯苯并 [a] �I;反式 -5,6- 環氧 -7- 氯苯并 [a] �I; 和 4-,5-,6-,8-,9- 羥基 - 7- 氯苯并 [a] �I。 反式 -3,4- 二氫二醇 -7- 氯苯并 [a] �I的代謝量,正常飲食之微 粒體代謝實驗較能量限制者高; 同時,代謝也發生在有公牛胸腺 DNA 的情況下,結果產生 修飾的 DNA,藉由�e P 後標記後, 薄膜層析和高壓液相層析儀來分離與分析,所有實驗中 只發現一個 DNA 加成物, 即是由反式 -3,4- 二氫二醇 -7- 氯苯并 [a] �I衍生的加成物。 相同性別和年齡的小鼠中,微粒體合成 DNA 加成物的量,能量限制的較正常飲食者低; 因 此, 我們的結果顯示能量限制抑制即將發生之代謝物 - 反式 -3,4- 二氫二醇 -7- 氯苯并 [a] �I的形成和明顯地降低其 DNA 加成物的形成。 |
英文摘要 | The effect of caloric restriction (CR) on the metabolism and DNA binding of 7-chlorobenz[a]anthracene (7-Cl-BA), a tumorigenic environmental contaminant, by liver microsomes of 9-month-old male B6C3F �� mice and 9- and 12-month-old female B6C3F �� mice was studied. Mice were fed ad libitum (AL), or starting at 14 weeks of age, received 60% of the calories consumed by control mice and were sacrified at 9 or 12 months of age. After microsomal incubations, metabolites were extracted, purified by reversed-phase HPLC, and identified by comparison of their UV-visible and mass spectral data with those of standards. In each case, ten metabolites were identified: 7-Cl-BA trans-3, 4-dihydrodiol, 7-Cl-BA trans-5, 6-dihydrodiol, 7-Cl-BA trans-8, 9-dihydrodiol, 7-Cl-BA trans-10,11-dihydrodiol, 7-Cl-BA 5,6-epoxide, and 4-,5-,6-,8-,and 9-OH-7-Cl-BA. The quantity of 7-Cl-BA trans-3,4-dihydrodiol was formed in a higher yield from the AL microsomes than from the CR microsomes. Metabolism was also performed in the presence of calf thymus DNA, and the resulting modified DNA was isolated and analyzed by both �e P-postlabeling/ TLC and �e P-postlabeling/HPLC. Only one DNA adduct, which was derived from 7-Cl-BA trans-3,4-dihydrodiol, was detected in all cases. The levels of adduct formation from CR microsomes were lower than those from the AL microsomes of the same sex and same age. Thus, our results indicate that CR inhibits the formation of the proximate metabolite, 7-Cl-BA trans-3,4- dihydrodiol, and significantly reduces the DNA adduct formation. |
本系統中英文摘要資訊取自各篇刊載內容。