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頁籤選單縮合
題名 | Effect of Different Anticoagulant, Underfilling of Blood Sample and Storage Stability on Selected Hemogram=抗凝固劑、抽血量不足及儲存穩定度對一般血液檢查之影響 |
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作者 | 陳百薰; 馮貞芬; 江建華; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 15:2 1999.02[民88.02] |
頁次 | 頁87-93 |
分類號 | 414.7 |
關鍵詞 | 抗凝固劑; 抽血量不足; 儲存穩定度; 血液檢查; Anticoagulant; Underfilling; Storage stability; |
語文 | 英文(English) |
中文摘要 | 為探討不同抗凝固劑、抽血量不足及儲存穩定度對血液檢查中血色素、血球比容 、 MCV、MCH、MCHC、白血球中性球及淋巴等項目之影響, 吾人總共收集 94 個高雄醫學院 附設醫院非血液性疾病門診及位院病患周邊血液,自動血液白血球五項分類分析儀使用日本 SYSMEX NE8000 型儀器。含 EDTA 抗凝固劑真空採血管使用 Becton-Dickinson 公司產品。 吾人研究 28 位以 Na �� EDTA 管收集者,結果顯示其血色素、血球比容、MCV 及淋巴球比 率等項目較高於以 K �� EDTA 管收集者 (P<0.05 ,P<0.01, P<0.01,P<0.05),而在 MCHC 項目則較低於以 K3EDTA 收集管者 (P<0.05)。針對抽血量不足之研究顯示 28 位以 2ml 抽 血量者其血球比容、 MCV 值低於以標準 5ml 抽血量者 (P 均 <0.01), 而當抽血量增加至 3.5 ml 時則 28 例病患其血液相則與 5ml 抽血量者無有意義之差異。在儲存穩定度研究上 ,對另外 10 例病患其血液經分別抽血 4 小時、8 小時、 12 小時後血比容及 MCV 較抽血 後一小時者呈現統計學上有意義之連續性上升 (與 8 小時差分別為 P<0.05 與 <0.01, 與 12 小時差 P 均 < 0.01), 中性球比率則在抽血後 4 小時、8 小時及 12 小時後均較抽血 後 1 小時者呈現統計學上有意義之連續性下降 (P 均 <0.01)。 |
英文摘要 | We collected blood samples from 94 adult non-hematological outpatients and inpatients for complete blood count (CBC) without any flagging at Kaohsiung Medical College Hospital in order to investigate the effect of (1) different anticoagulants with Na �� EDTA vs K �� EDTA (2) the underfilling of blood collection volume (2 ml, 3.5 ml vs standard 5 ml) (3)the difference in storage stability between 1 hour, 4 hours, 8 hours and 12 hours after venesection at room temperature on some selected hemogram parameters (WBC, RBC, hemoglobin, hematocrit, MCV, MCH, MCHC, platelet, percentage of neutrophil and lymphocyte). The automated hematology analyzer we used was SYSMEX NE-8000, (TOA, Japan). All the EDTA collection vacutainer tubes were supplied by Becton-Dickinson (New Jersey, U.S.A.) with the same lot number. Paired t-test was used for statistics. We found that values of hemoglobin, hematocrit, MCV and lymphocyte percentage collected in Na �� EDTA tubes were significantly higher than those collected in K �� EDTA (P<0.05 for hemoglobin and lymphocyte percentage, and P all<0.01 for others), while values of MCHC collected in Na �� EDTA were significantly lower than those collected in K �� EDTA (P <0.05). For underfilling of blood sample, values of hematocrit and MCV with 2 ml blood volume were significantly lower than those with 5 ml blood volume (both P< 0.01), while values of MCHC with 2 ml blood volume were significantly higher than those with 5 ml blood volume (P<0.01). When the collection blood volume was increased to 3.5 ml, there were no significant difference between values for 3.5 ml and 5ml blood volume (P all>0.05). In the storage stability study, there was a significant sequential increase of hematocrit and MCV between 1 hour, 8 hours and 12 hours (P<0.05 and <0.01, respectively, for 8 hours, P all<0.01 for 12 hours). There was also a significant sequential decrease of neutrophil percentage between 1 hour and 4, 8, 12 hours' storage at room temperature (P all<0.01). |
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