頁籤選單縮合
題 名 | Identification of Oligo-N-glycolylneuraminic Acid Residues in Mammal-derived Glycoproteins by a Newly Developed Immunochemical Reagent and Biochemical Methodsd |
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作 者 | Sato,Chihiro; Kitajima,Ken; InoueSadako; InoueYasuo; | 書刊名 | 中央研究院生物化學研究所論文集 |
卷 期 | 24 1998[民87.] |
頁 次 | 頁211-218 |
分類號 | 361.19 |
關鍵詞 | Oligo-N-glycolylneuraminic acid residues; Mammal-derived glycoproteins; Oligo-neu5Gc; |
語 文 | 英文(English) |
英文摘要 | The occurrence of the α2→8-linked oligomeric form of N- glycolyliieuraminic acid (oligo-Neu5Gc) residues in mamnialian glycoproteins was unequivocally demonstrated using a newly developed anti-oligo/poly-Neu5Gc monoclonal antibody as well as by chemical and biochemical methods. First, the antibody, designated mAb.2-4B, which specifically recognized oligo/polyNeu5Gc with a degree of polymerization of >2, was developed by establishing a hybridoma cell line from P3UI myeloma cells fused with splenocytes from an MRL autoimmune mouse immunized with dipalmitoylphos-phatidylethanolamine-conjugated oligo/poly-Neu5Gc. Second, oligo-Neu5Gc was shown to occur in glycoproteins derived from pig spleen by Western blot analysis using mAb.2-4B, which was also confirmed by fluorometric high performance liquid chromatographic analysis of the product of periodate oxidation/reduction/ acid hydrolysis of the purified glycopeptide fractions and by TLC and 600-MHz �� H NMR spectroscopic analysis of their mild acid hydrolysates. Finally, the ubiquitous occurrence of oligo-Neu5Gc chains as glycoproteinaceous components in Wistar rat tissue was immunochemically indicated. This is the first example demonstrating the diversity in oligo/poly-Sia structure in mammalian glycoproteins, where only poly-n-acetyl-neuraminic acid is known to occur. Such diversity in oligo/poly-Sia structure also implicates a diverged array of biological functions of this glycan unit in glycoproteins. |
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