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頁籤選單縮合
題名 | 產蛋下降症病毒臺灣分離株基因體之限制酶分析=Differentition of Egg Drop Syndrome Virus in Taiwan Isolates by Restriction Endonuclease Analysis |
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作者 | 林德田; 賴秀穗; 許天來; 林地發; 楊華章; 蘇杰夫; 林士鈺; | 書刊名 | 臺灣省家畜衛生試驗所研究報告 |
卷期 | 34 1998.12[民87.12] |
頁次 | 頁31-41 |
分類號 | 437.246 |
關鍵詞 | 產蛋下降症; 限制酶; Egg drop syndrome; Restriction endonuclease; |
語文 | 中文(Chinese) |
中文摘要 | 臺灣分離產蛋下降症(Egg drop syndrome, EDS)病毒CO株DNA經限制�tSal Ⅰ、 EcoR Ⅰ、BamHI、Cla Ⅰ、Xho Ⅰ、Kpn Ⅰ、Hind Ⅲ、Pvu Ⅱ、Pst Ⅰ、及 Sma Ⅰ切割並 在 1% 和 2 % agarose 下電泳分析,結果顯示出DNA切割片段數依序分別為2個、4個、4個、3個 、4個、5個、10個、11個、9個及4個。同時利用限制�t切割分析臺灣雞及鴨各EDS 病毒株DNA差異性,結果顯示7株病毒(NO.4、NO.5、NO.7、NO.8、NO.18、CO、JAP- 1)中有2株與其他5株有顯著的差異,尤其NO.4分離株經PstⅠ、SalⅠ、SmaⅠ、ClaⅠ 及HindⅢ等限制�t切割分析後,均比其他毒株多出一個核酸片段,由此得知EDS病毒 在鴨與雞分離株之病毒DNA在限制�t切割分析上有顯著的差異,另EDS CO病毒株限 制�t切割圖譜與JAP-1病毒則相同。又將EDS-76CO分離株與人類腺病毒第2型及家禽 腺病毒NO.9株進行HindⅢ限制�t切割比較分析結果,可發現三種腺病毒的核酸限制�t 切割圖譜之核酸片段數與分子大小完全不同。為探討EDS-76 NO.4病毒株DNA經Hind Ⅲ核酸限制�t切割分析所多出一核酸片酸片段之特異性,再利用Digoxigenin(DIG)標示NO.4 與CO病毒DNA,合成核酸探針進行交叉南方轉漬試驗,結果證實二種探針均能檢測 出約3.97kb核酸片段。因此,3.97 kb核酸片段屬於EDS-76之基因體DNA。 |
英文摘要 | Egg drop syndrome virus (EDSV) DNA of a Taiwan chicken isolate, the CO strain, was analysed and cleavaged by ten endonucleases, ie., SalⅠ, BamHI, Cla Ⅰ, Xho Ⅰ, kpn Ⅰ, Hind Ⅲ, Pvu Ⅱ,Pst Ⅰ and Sma Ⅰ. DNA fragments were analyzed and electrophoresed in the 1% and 2% agarose. The numbers of fragments were found 2 (Sal Ⅰ ), 4(EcoRI), 4(BamHI), 3( Cla Ⅰ ),4(Xho Ⅰ ), 5(Kpn Ⅰ ),10 (Hind Ⅲ ), 11 (Pvu Ⅱ ),9(Pst Ⅰ ), 4(Sma Ⅰ ), respectively. Seven EDSV isolates from chicken and ducks (NO.4, NO.5,NO.7, NO.8, NO.18, CO and JAP-1), including six Taiwan isolates and one Japan isolate (JAP-1), were compared by endonuclease analysis of virus DNA. Two of them were showed the distinctive difference from other five isolates, especially the strain NO.4 which having one extra DNA fragment (3.97 kb) with Pst Ⅰ, Sal Ⅰ,Sma Ⅰ,Cla Ⅰ and Hind Ⅲ. On the other hand, the DNA fragment pattern of the CO strain was showed to be identical to that of the JAP-1 strain. Comparing the DNA fragment patterns treated with Hind Ⅲ, the EDSV CO strain, human adenovirus serotype 2 and avian adenovirus (No.9) were showed difference. For further study the extra DNA fragment of the CO strain treated by Hind Ⅲ,two digoxigenin-labled probes from the NO.4 and CO strain genomic DNA were made. Using both probes, the extra 3.97 Kb DNA fragment in NO.4 strain were detected by corss southern blotting technique. This indicated that homologous DNA sequence was present between EDS strains of CO and NO.4 |
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