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題名 | 斜紋夜蛾核多角體病毒化學增效作用之研究=Enhancement Effects of Chemical Additives on the Virulence of Spodoptera Litura Nucleopolyhedrovirus |
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作者 | 石正人; 李淑增; Shih, Cheng-jen; Lee, Shu-tseng; |
期刊 | 中華昆蟲 |
出版日期 | 19981200 |
卷期 | 18:4 1998.12[民87.12] |
頁次 | 頁273-283 |
分類號 | 433.3 |
語文 | chi |
關鍵詞 | 斜紋夜蛾核多角體病毒; 增效作用; 病毒殺蟲劑; 蟲害防治; Spodoptera litura nucelopolyhedrovirus; Enhancement; Viral insecticides; Insect control; |
中文摘要 | 本文測試多種化學藥品添加劑,評估其對斜紋夜蛾(Spodoptera litura)核多角體病毒致病力之增強效果。測試五種不同核多角體病毒,得知對斜紋夜蛾幼蟲致病力最強的病毒為分離自臺灣的斜紋夜蛾核多角體病毒。選取數種藥品包括剛果紅(congo red)、山梨酸(sorbic acid)、硼酸(boric acid)、卵磷脂(lecithin)、磷酯醯乙胺醇(phosphoatidyl ethanolamine )、磷酯醯膽鹼(phosphoatidyl choline)、螢光增白劑(leucophor)及螢光增亮劑(fluorescent brightener 28)等,進行其對斜紋夜蛾核角體病毒增效作用之測試。結果在細胞培養液中分別添加上述幾種化種藥品,僅在添加 0.01% 之劑量時,細胞方可維持正常生長。利用細胞培養方法測試各種化學藥品對病毒感染細胞之增效作用,結果添加0.01%之sorbic acid及boric acid可以降低TCID�t。在斜紋夜蛾幼蟲的活體試驗中,取純化之斜紋夜蛾核多角體病毒,與數種供試化學增效劑分別混合後, 結果增效作用較強者有 leucophor、fluorescent brightener 28、lecithin、 phosphatidyl choline、及phosphatidyl ethanolamine等五種化學藥品。除fluorescent brightener 28 外,四種化學藥品的濃度對增效作用之效果呈正相關。 |
英文摘要 | This paper presents, a study on enhancers for Spodoptera litura nucleopolyhedrovirus (SlNPV). In order to enhance the virulence of SlNPV, several chemicals, eg., congo red, sorbic acid, boric acid, fluorescent brightener 28, leucophor, lecithin, phosphatidyl choline, and phosphatidyl ethanolamine, were respectively mixed with the virus suspension to evaluate the efficacy of enhancement. We tested 5 different species of NPVs to check their efficacy. The results revealed that SlNPV was the most effective for infecting S. litura larvae. When we added chemicals into the cell culture medium, TNM-FH, the SL7B cell line could grow only when the concentration was 0.01%. Consequently, we investigated the TCID�tof SlNPV B strain ECV with or without 0.01% chemical addition. Results revealed that several chemicals could reduce the TCID�t of SlNPV B strain ECV. However, larvae fed on artificial diet containing 1% of different chemicals showed no adverse effects. Bioassay revealed that an artificial diet surface-contaminated with virus suspension contained 1% of different chemicals could increase the mortality of S. liture larvae as compared to virus suspension only. The mortality of S. litura larvae increased with increasing concentration of chemicals added in the virus suspension except for fluorescent brightener 28. |
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