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題名 | 飲用水中酚類化合物之分析及多氯酚導致DNA鏈斷裂之研究= |
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作者 | 王應然; 林仁混; Wang, Yen-zan; |
期刊 | 康寧學報 |
出版日期 | 19981200 |
卷期 | 1:1 1998.12[民87.12] |
頁次 | 頁171-185 |
分類號 | 412.31 |
語文 | chi |
關鍵詞 | 飲用水; 酚類化合物; 多氯酚; |
中文摘要 | 針對自然水及飲用水中的酚類化合物,使用檢體中直接乙醯化的技術並以氣相層 析質譜儀之選擇性離子偵測去( GC ╱ MS-SIM )做為分析工具,水中酚類化合物濃度僅須 每公升含有數 ng 即可被偵測。此分析方法的優點在於具有較佳的回收率及較低的基質干擾 。實際分析臺灣四個自來水廠的酚類化合物含量其濃度在每公升含 12 ∼ 312ng 之間。 在眾多的酚類化合物中,2,4,6﹣三氯酚,2,3,4,6﹣四氯酚及五氯酚已被證實為致癌物質。 利用小白鼠胚胎纖維母細胞( C3H10T1 ╱ 2 )以 DNA 沈澱試驗檢測此類多氯酚在肝臟活 化系統存在或不存在時對 DNA 的損害程度, 發現這三種多氯酚在肝臟活化系統存在時可引 起 C3H10T1 ╱ 2 細胞內少量單股 DNA 的斷裂。 當以五氯酚的代謝產物四氯對苯二酚做同 樣的測試時發現不需要肝臟活化系統的存在即可造成明顯的單股 DNA 斷裂。 此結果顯示, 當評估此類多氯酚的致癌潛能時,其代謝產物須列入考慮。由多氯酚的代謝產物造成之 DNA 鏈的斷裂可能在致癌過程中扮演重要的角色。 |
英文摘要 | Gas chromatography/mass spectrometry with selected-ion monitoring (GC/MS-SIM) method were used to analyze 23 selected phenols in natural and drinking waters by an in situ acetylation technique. This method was suitable for determining phenol concentrations at the ng/L level because of less background interference and better recoveries. The application range for all these phenols was from 0.01 or 0.04 to 10 μ g/L, using a 800 ml water sample. The levels or trace phenols in four Taiwan water treatment plants were in the range of 12-213 ng/L. The polychlorinated phenols, namely 2, 4, 6-trichlorophenol, 2, 3,4,6-tetrachlorophenol, and pentachlorophenol, were evaluated for their ability to induce deoxyribonucleic acid (DNA) damage using a DNA precipitation assay employing mouse embryonic fibroblast cells (C3H10T1/2) with or without a liver microsomal activation system. These agents exhibited a weak positive response when microsomal activation enzymes were present in this assay. When the tetrachlorohydroquinone, a toxic metabolite of pentachlorophenol, was measured by the same method without the activation system, a significant and dose-dependent DNA damage was found. This result indicates that in the evaluation of the carcinogenic potential of these agents, their corresponding metabolites should be taken into consideration. DNA strand breakage caused by these active metabolites may play an important role in the tumorigenetic process of polychlorinated phenols. |
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