查詢結果分析
來源資料
相關文獻
- Enzymatic Characterization of Three Entomopathogenic Fungi with the API ZYM System
- Sensitivity of Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae to Selected Fungicides
- 黑殭菌抗殺菌劑菌株之篩選
- Efficacy of Metarhizium Anisopliae and Beauveria Bassiana against Helicoverpa Armigera in Chickpea, under Field Conditions in Nepal
- Virulence of Metarhizium Anisopliae and Beauveria Bassiana against Common White Grubs in Nepal
- Virulence of Native Isolates of Metarhizium Anisopliae and Beauveria Bassiana against Helicoverpa Armigera in Nepal
- 本土黑殭菌素對甜菜夜蛾幼蟲之毒效探討
- 本土黑殭菌以固態和液態發酵生產黑殭菌素之探討
- 利用蟲生真菌 (黑殭菌) 防治青葱甜菜夜蛾
- Evaluation of Beauveria bassiana for Controlling Casuarina Tussock Moth (Lymantria xylina Swinhoe) in Casuarina Plantations
頁籤選單縮合
題名 | Enzymatic Characterization of Three Entomopathogenic Fungi with the API ZYM System=以API ZYM系統測定蟲生真菌之酵素特性 |
---|---|
作者 | 蕭文鳳; | 書刊名 | 中華昆蟲 |
卷期 | 18:3 1998.09[民87.09] |
頁次 | 頁203-206 |
分類號 | 379.1 |
關鍵詞 | 白殭菌; 黑殭菌; 蠟蚧幹枝孢菌; Beauveria bassiana; Metarhizium anisopliae; Verticillium lecanii; API ZYM; |
語文 | 英文(English) |
中文摘要 | 本實驗利用半定量之API ZYM 系統來研究三種蟲生真菌,白殭箘Beauveria bassiana ,黑殭菌 Metarhizium anisopliae,蠟蚧幹枝孢菌Verticillium lecanii的酵素剖圖。所有的分離株對β-glucuronidase 及cysteine aminopeptidase 的基質無反應,但黑殭菌195菌株對後者有反應。所有的分離株對Lipase, trypsin, chymotrypsin, α-glucos idase 及α-fucosidase 的基質無反應或只有輕微的反應。而白殭菌 Col 42品系對α-mann osidase基質有很強的反應。 黑殭菌的二個分離株(194及195)對leucine aminopeptidase, phosphatase acid, phosphoamidase, β-glucosaminidase具有強烈之反應。除此外,黑殭菌194對esterase, esterase lipase和β-glucosidase 的基質反應較強而黑殭菌195則只有中度的反應。就繼代培養而言,白殭菌第九代之培養對phosphatase alkaline, leucine aminopeptidase, α-galatosidase, β-galactosidase的基質反應活性會下降。蠟蚧幹枝孢菌第六代之培養對leucine aminopeptidase, valine aminopeptidase, phosphoamidase, β-glucosidase,及β-glucosaminidase反應活性會下降。相反的對phosphatase alkaline 反應有些微上升。以上的結果顯示API ZYM測試方法可提供快速的半定量的方法來辨識不同分離株及繼代培養的酵素活性。 |
英文摘要 | The enzymatic profiles of Beauveria bassiana (Bb), Metarihizium anisopliae (Ma), Verticillium lecanii (VI), and their subcultures were studied by using the API ZYM system. Isolates all showed no reaction to substrates with β-glucuronidase, or cystein aminopeptidase except for Ma (str. 195). All isolates showed little sctivity or were completely without activity to substrates with lipase, trysin, chymotrysin, α-glucosidase, orα-fucosidase. However, Bb Ho 42 reacted strongly to substrate with α-mannosidase. Both isolates of M. anisopliae (strs. 194 and 195) reacted strongly to substrates with leucine aminopeptidase, acid phosphatase, phosphoamidase, orβ-glucosaminidase. Additionally, M. anisopliae str. 194 reacted strongly to the substrates with esterase (C4), or esterase lipase, while M. anisoplaie str. 195 moderately reacted to the substrates with β-glucosidase. Results showed that the enzymatic activity of B. bassiana against substrates with phosphatase alkaline, leucine aminopeptidase, α-galactodase, orα-galactodase declined after serial passages from subcultures of Ho. 212 F3 to F9. V. lecanii exhibited the tendency of declining activity against substrates with leucine aminopeptidase, valine aminopeptidase, phosphosamidase, β-glucosidase, orβ-glucosaminidase from subcultures of F4 to F6. On the other hand, the reaction to the substrate with phosphatase alkline increased. The results of this study indicate that API ZYM techniques provide a rapid and reproducible semi-quantitative method for revealing enzymatic activity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。