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題 名 | Evaluation of the Non-Radioactive Detection of the CAG Repeats in the Machado-Joseph Disease Gene=非放射性的Machado-Joseph疾病篩選方式之評估 |
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作 者 | 吳明娟; 周隆武; 王敏英; | 書刊名 | 嘉南學報 |
卷 期 | 24 1998.11[民87.11] |
頁 次 | 頁45-51 |
分類號 | 415.135 |
關鍵詞 | 非放射性; Machado-Joseph疾病; 篩選方式; Triplet repeat; PCR; MJD; |
語 文 | 英文(English) |
中文摘要 | Machado-Joseph 疾病 (MJD) 是由於位在染色體 14q32.1 的 MJD基因的 CAG 三 核甘酸不正常延長所造成。 在這篇報告中我們評估一個非放射性 PCR 結合洋菜膠電泳的 MJD 篩選方式,此法的靈敏度及正確性與放射或 PCR 結合聚丙烯醯氨電泳相當,只要 25ng 的染色體 DNA,CAG 的重複次數即可以被正確的檢驗出來,因此此法可供一般醫療檢驗單位 篩選高危險群之用。 |
英文摘要 | Machado-Joseph disease (MJD) is associated with the expansion of a CAG triplet in a novel gene on 14q32.1. We evaluated a non-radioactive detection method for the screen of MJD. This non-radioactive PCR combined with agarose gel electrophoresis provides a comparably sensitive and accurate detection method as radioactive PCR with polyacrylamide gel electrophoresis. As low as 25 ng of genomic DNA can be specifically amplified by PCR and the numbers of CAG repeat can be determined by agarose gel electrophoresis. This safe and quick detection method can by used in routine clinical laboratories for screening hith-risk population of MJD. |
本系統中英文摘要資訊取自各篇刊載內容。