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頁籤選單縮合
題名 | cDNA Cloning and Expression Analysis of the Human UDPglucose Dehydrogenase=人類尿嘧啶雙磷酸葡萄糖去氫酶之cDNA選殖與表現分析 |
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作者 | 彭慧玲; 羅明德; 張梅玲; 張晃猷; | 書刊名 | Proceedings of the National Science Council : Part B, Life Science |
卷期 | 22:4 1998.10[民87.10] |
頁次 | 頁166-172 |
分類號 | 361.4 |
關鍵詞 | 肝臟; 人類尿嘧啶雙磷酸葡萄糖去氫酶; cDNA選殖; Molecular cloning; Gene expression; UDPglucose dehydrogenase; |
語文 | 英文(English) |
英文摘要 | A cDNA clone encoding the human UDPglucose dehydrogenase was isolated from a liver cDNA library. The cDNA is 2,355 bp in length with an open reading frame which is capable of encoding a protein of 494 residues. The predicted primary sequence of the gene product is in good agreement with that of the bovine enzyme determined previously found by means of protein sequencing. Two major transcripts of the UDPglucose dehydrogenase gene with sizes of 2.8 and 2.35 kb, respectively, were observed by Northern analysis. The gene was found to be ex pressed in a variety of tissues with the highest level in liver, consistent with the physiological function of the enzyme in excretion of endo- and xenobiotics compounds. |
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