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題 名 | 以PCR之普遍性引子增殖混合酵母菌體的核醣體RNA基因之靈敏度=Effect on Sensitivity of PCR Amplification of Ribosomal DNA Gene Using Universal Primer with a Mixture of Yeast Species |
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作 者 | 黃錦城; 蔡維鐘; 王西華; | 書刊名 | 藥物食品分析 |
卷 期 | 6:3 1998.09[民87.09] |
頁 次 | 頁579-586 |
分類號 | 364.6 |
關鍵詞 | 聚合酶鏈鎖反應; 普遍性引子; 核醣體RNA基因; 酵母菌; 內轉錄區; Polymerase chain reaction; Universal primer; Ribosomal RNA gene; Yeast; Internal transcribed spacer; |
語 文 | 中文(Chinese) |
中文摘要 | 使用PCR之普遍性引子增殖酵母菌之核糖體RNA基因之內轉錄區,以檢測單一或 混合二種或三種酵母菌之空敏度。以Novozyme分解三個屬之酵母菌的細胞壁抽取DNA,DNA 的抽取量隨茵種不同而有很大的差異。以一對普遍性PCR引子增殖單一和混合酵母菌的核醣 體RNA基因的內轉錄區ITS I,PCR之靈敏度在Saccharomyces exiguus為2.5pg的抽取DNA(約 10�揚茞茩M),Candida mogii為12pg(約10�蘊茞茩M),Saccharomyces cerevisae為20pg(約 10�蘊茞茩M),由於三株菌經由PCR增殖產物的片段長度皆不同,因此以PCR增殖二種或三 種混合酵母菌之DNA,在同樣PCR條件下,其靈敏度較單一酵母菌降低約1000倍(約10��- 10�瀕10個細胞)。 |
英文摘要 | To detect the sensitivity of PCR using universal primers to amplify the RNA gene of internal transcribed spacer (ITS I with a mixture of yeast species. The amounts of extracted DNA obtained from three yeast genera using Novozyme to lyse the cell walls differed significantly. When a set of universal primers were used in pure yeast cultures, PCR sensitivity for Saccharomyces exiguus was 2.5 pg (approx. 10�� cells), for Candida mogii 12 pg (approx. 10�� cells), and for Saccharomyces cerevisiae 20 pg (approx. 10�� cells). In mixed yeast cell cultures, there was a 1000-fold (approx. 10��-10�瀕10 cells) decrease in sensitivity under the same PCR conditions. |
本系統中英文摘要資訊取自各篇刊載內容。