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題名 | Effect of Trilinolein on Superoxide Dismutase Activity in Isolated Rat Cardiomyocytes Subjected to Hypoxia and Normoxic Perfusion=三亞麻油酸在大白鼠心肌細胞處於缺氧及再灌流狀態下對於超氧化物轉化酵素的影響 |
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作者 | 劉如濟; 陳保羅; 鄭瑞棠; 陳建平; 李相臺; 張念中; Liu, Ju-chi; Chan, Paul; Cheng, Juei-tang; Chen, Jane-pyng; Lee, Shiang-tai; Chang, Nen-chung; Tomlinson,Brian; |
期刊 | Acta Cardiologica Sinica |
出版日期 | 19980400、19980500、19980600 |
卷期 | 14:2 民87.04-06 |
頁次 | 頁52-57 |
分類號 | 418.11 |
語文 | eng |
關鍵詞 | 氧自由基; 缺氧; 再灌流; 超氧化合物轉化酵素; 三亞麻油酸; Free radicals; Hypoxia; Reperfusion; Superoxide dismutase; Trilinolein; |
中文摘要 | 背景:心肌細胞於缺氧/再灌流狀態下會受到氧自由基的破壞,過去文獻顯示內生性超氧化合物轉化酵素的作用減少與氧自由基產生的破壞有相關性。而有些物質可以清除這些氧自由基以達到心肌保護的作用。三亞麻油酸是一粹取自中國傳統中草藥(三七)的強力植物性抗氧化劑,已被証實具有減少心肌於缺氧狀態下的破壞。本研究即探討三亞麻油酸在缺氧/再灌流狀態下,對於內生性超氧化合物轉化酵素的影響。 方法:分誰出兩個月大的大白鼠心肌細胞後,以100%氧氣供給分雖心肌細胞液中10分鐘產生(供氧期),繼以100%氮氣注入產生(缺氧期),之後再以100%氧氣注入產生所謂的(再灌注期)。在不同時期,測量兩種不同的超氧化合物轉化酵素的活性(銅、鋅-超氧化合物轉化酵素;錳-超氧化合物轉化酵素)。然後給予不同濃度的三亞麻油酸,再測量這兩種酵素的活性,並比較共變化。 結果:在給予三亞麻油酸後,錳-超氧化合物轉化酵素的活性,於不同的時期呈現有意義的增加。對於銅、鋅-超氧化合物轉化酵素而言,在高濃度的三亞麻油酸情況下,可在(供氧期)及(再灌注期)增加此酵素的活性,而(缺氧期)的活性則低於對照組。 結論:三亞麻油酸為一有效的氧自由基清除物質,它對心肌細胞的保護作用可經由直接與氧自由基作用,亦可透過超氧化合物轉化酵素系統來間接影響氧自由基。 |
英文摘要 | Background:Myocardial damage during ischemia/reperfusion has been linked to oxygen free radical production. Scavengers of oxygen free radicals can effectively ameliorate myocardial injury during ischemia/reperfusion. Decreased endogenous SOD activity may contribute to free-radical-mediated reperfusion injury of the ischemic myocardium. Trilinolein is a potent natural plant antioxidant recently purified from the traditional Chinese medicinal plant Panax pseudoginseng. It has linoleic-acid residues as the only type of fatty acid in all three esterified positions of the triacyiglycerol. Methods and Results: The present data reveal that, when isolated rat cardiomyocytes were pretreated with 10□ M trilinolein, after oxygenation with 100% O□ for 5 min, the activities of Mn-SOD and Cu.Zn-SOD were potentiated. During hypoxia, in cardiomyocytes pretreated with 10□ and 10□ M trilinolein, the activity of Mn-SOD was reduced but was still greater than the control levels without trilinolein, whereas the rise in Cu.Zn-SOD seen with control studies was alternated. During subsequent normoxia cardiomyocytes pretreated with trilinolein showed further decreases in Mn-SOD whereas the controls tended to rise. Cu.Zn-SOD levels were maintained compared with a fall in control studies. Conclusions: Since ischemia/reperfusion produce excessive amount of oxygen free radicals when compared to ischemia only, the preservation of Cu.Zn-SOD activity in isolated cardiomyocytes after pretreatment with trilinolein implies trilinolein is an effective free radical scavenger which could stabilize the levels of SOD during hypoxia and subsequent normoxia. |
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