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題名 | 乳酸桿菌科菌株提高免疫力及抗癌方面的研究=The Sutdy of Immunopotentiating and Anti-Tumor Abilities of Lactobacteriaceae |
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作者姓名(中文) | 羅敏菁; 季匡華; | 書刊名 | 放射治療與腫瘤學 |
卷期 | 5:2 1998.06[民87.06] |
頁次 | 頁111-117 |
分類號 | 369.35 |
關鍵詞 | 乳酸桿菌科; 免疫增強作用; 抗癌; 遲發性過敏反應; 佐劑; Lactobacteriaceae; Immunopotentiation; Anti-tumor; Delayed type hypersensitivity; Adjuvant; |
語文 | 中文(Chinese) |
中文摘要 | 目的:測試乳酸桿菌科菌株提高免疫力之效果及其抗癌方面的表現。 材料與方法:本研究以不同來源之乳酸桿菌科死菌體及靈芝萃取液,能否在體外刺激老 鼠脾臟細胞產生γ—擾素,做為其增強免疫力的指標;再以遲發性過敏反應(Delayed Type Hypersensitivity; DTH)測試乳酸桿茵科菌株的佐劑作用及其引發的T細胞記憶反應;最後 以同時注射方式,測定乳酸桿菌科死菌體抑制肉瘤細胞(CT-26)的生長情形,並與BCG比較 之。 結果:對體外培養的老鼠脾臟細胞,所有的乳酸桿茵科死菌體在刺激γ—擾素的生成作 用上都比靈芝強,而比Con A稍差。同時施打羊紅血球(SRBC)和乳酸桿茵科死菌體時,可以 稍微增加皮膚的腫脹程度,表示它對遲發性過敏反應有輕度的佐劑作用。而先打死菌體刺激 局部的免疫系統,再以SRBC免疫時,局部的遲發性過敏反應要比未打死菌體的對照組強。 另外同時注射死菌體與腫瘤細胞時,死菌體比BCG活菌更能抑制肉瘤細胞的生長。 結論:經本實驗獲得初步的證實,乳酸桿菌科菌株可增強免疫反應並抑制癌細胞的生長、 但其完整的作用機轉,還須進一步深入研究。 |
英文摘要 | Purpose: To examine the immunopotentiating ability of Lactobacteriaceae with Ganoderma lucidum in vitro and compare the anti-tumor ability of Lactobacteriaceae with that of BCG using a mouse model injected with sarcoma cells. Materials and Methods: The immunopotentiating ability was tested in vitro using the stimulation of interferon-gamma production by mouse spleen cells. The delayed type hypersensitivity shown by foot swelling scale was chosen as an index of adjuvant effect. Anti-tumor ability was examined by simultaneously injected bacteria cells with sarcoma cells on mouse left hind leg. Results: The concentrations of interferon-gamma proliferation by various heat-killed Lactobacteriaceae cells were higher than four tested Ganoderma lucidum products, but slightly lower than Con A. Simultaneously injected SRBC with heat- killed Lactobacteriaceae cells showed an adjuvant effect on foot swelling in mouse model. Mice immunized with heat-killed Lactobacteriaceae cells had higher foot swelling scale when challenged with SRBC three weeks later. Heat-killed Lactobacteriaceae cells demonstrated a better inhibition of tumor growth than viable BCG. Conclusion: The immunopotentiating and anti-tumor abilities of Lactobacteriaceae are initially verified, further studies should be carried on. |
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