頁籤選單縮合
題名 | 小耳種李宋系迷你豬緊迫敏感基因之篩選=Selection of Stress Susceptible Genotype of the Small-Ear Lee-Sung Strain Miniature Pig |
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作者 | 王佩華; 宋永義; 張直; 鄭位明; Wang, Pey-hwa; Sung, Yung-yi; Chang, Tse; Cheng, Wei-ming; |
期刊 | 中國畜牧學會會誌 |
出版日期 | 19980300 |
卷期 | 27:1 1998.03[民87.03] |
頁次 | 頁35-48 |
分類號 | 437.654 |
語文 | chi |
關鍵詞 | 李宋系迷你豬; 豬緊迫敏感徵候群; 鹵乙烷檢測; 鈣離子釋放管道; Lee-sung strain miniature pigs; Porcine stress syndrome; PSS; Halothane test; Calcium release channel; CRC; |
中文摘要 | 本試驗旨對小耳種李宋系迷豬利用鹵乙烷法及聚合脢鏈鎖反應配合限制內切脢分 析( PCR-REA )檢測豬鈣離子釋放管道( CRC )基因 1843 鹼基是否突變之兩種方法來判 定遺傳疾病-豬緊迫敏感徵候群( porcine stres syndrome, PSS )。 本試驗對國立臺灣 大學農業試驗場畜牧組場內 46 頭體重 15 ∼ 20 kg 李宋系迷你豬進行 3% 鹵乙烷氣體麻醉 檢測, 結果顯示在所檢測的 46 頭豬隻中,有 7 頭豬隻呈鹵乙烷陽性反應(其中一頭為疑 似陽性反應),約佔受測豬隻的 15.22%,此結果相較其他品種之陽性反應率為高。由對 11 頭小耳種豬及 84 個李宋系迷你豬進行 PCR-REA 檢測豬 CRC 基因 1811 至 1877 核甘酸序 列的試驗, 結果顯示,在所檢測的豬隻樣本中,經 Hha I 或 BsiHKA I 限制內切脢分切後 ,呈現兩種分切型,其中僅有一頭小耳種豬所出現的分切型為具 33/34 及 67 bp 兩條環帶 ( Hha I )的雜合基因型( C1843T ), 餘 94 個不論是小耳種及李宋系迷你豬所顯示的 分切型皆為具 33/34bp 一條環帶之正常型。另由利用 PCR 所複製的產物經核甘酸序列定序 及比對結果顯示,所得 67bp 產物確為豬 CRC 基因 1811 至 1877 核甘酸序列。 |
英文摘要 | The purpose of the study was to determine whether the samll-ear Lee-Sung strain miniature pig has genetic disorder - porcine stress sundrome (PSS) - by halohane test and polymerase chain reaction - restriction endonuclease assay (PCR-REA) to detectmutation at 1843 base of calcium release channel (CRC) gene. Forty-six Lee-Sung strain miniature pigs weighing 15~20 Kg were tested with 3% halothane gas. The results indicated that seven of forty-six head of the pigs were halothane positive reactors. The positive percentage was 15.22%, which was higher than the other breeds. Eleven small-ear and eighty-four Lee-Sung strain miniature pigs were detected for nucleotide 1811 to 1877 sequence of CRC gene by PCR-REA. The results indicated there were two patterns which clove with Hha I or BsiHKA I, and only one small-ear pig;s cleaving pattern was of the heterozuyote type which had 33/34 and 67 bp two bands (BsiHKA I site created by the C1843T exchange). The other pigs, either small-ear or Lee-Sung miniature strain pigs wrer normal type which has one band-33/34 bp. When PCR product was sequenced; the 67 bp fragment of PCR product was uncleotide 1811 to 1877 sequence of CRC gene reported by Fujii et al. (1911). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。