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題 名 | A Quick Method to Extract DNA from Paraffin-Embedded Tissues=由石蠟包埋組織萃取去氧核醣核酸的快速方法 |
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作 者 | 曾振淦; 曾雁明; 高淑岑; 陳素雲; 陳玉萍; | 書刊名 | 長庚醫學 |
卷 期 | 21:1 1998.03[民87.03] |
頁 次 | 頁63-66 |
分類號 | 364.21 |
關鍵詞 | 萃取去氧核醣核酸; 石蠟包埋組織; DNA extraction; Paraffin-embedded tissue; |
語 文 | 英文(English) |
中文摘要 | 背景:聚合酵素鏈反應、增進了分子生物學的應用;而去氧核醣核酸的萃取,提 供了利用此種技術的基本原料。在回溯性的研究 ,經由石蠟包埋組織塊萃取去氧核醣核 酸以利分析進行是常用的步驟。 方法:使用加熱冷卻的方法去除石蠟,蛋白溶解酵素加速溶出去氧核醣核酸,及利用濾膜 純化去氧核醣核酸。 結果:利用聚合酵素鏈反應的結果,顯示我們改良式的方法可以達成上述的目的,萃取得 的去氧核醣核酸量並不比傳統的方法遜色。 結論:改良式的方法使過程簡化而節省所需時間,並且無損於去氧核醣核酸的萃取。 |
英文摘要 | Background: The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA. Methods: We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method. Results: The amplified products from our method were superior to that of the conventional method. Conclusion: The method we propose has a better recovery of DNA and is more time efficient. |
本系統中英文摘要資訊取自各篇刊載內容。