頁籤選單縮合
| 題 名 | Simultaneous Determination of Paraquat and Diquat in Urine by Capillary Electrophoresis=毛細管電泳分析法同時定量尿中paraquat及diquat |
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| 作 者 | 吳文陞; 蔡錦蓮; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
| 卷 期 | 14:2 1998.02[民87.02] |
| 頁 次 | 頁76-80 |
| 分類號 | 414.91 |
| 關鍵詞 | 毛細管電泳分析法; Capillary zone electrophoresis; Herbicide; Solid phase extraction; |
| 語 文 | 英文(English) |
| 中文摘要 | 本文以報告以毛細管電泳 (CZE) 分析法定性及定量尿中paraquat(PQ) 及diquat(DQ)。paraquat及diquat是農業常用的除草劑,也是在醫院急珍室常見的自殺病人使用的毒劑,不幸的是PQ中毒沒有專屬的解毒劑,唯有靠血液或腹膜透析才能將體內毒素析出,所以建立一套快速可定性及定量的方法是非常迫切需要。本文利用在酸性緩衝液 (pH3.0;由10 mM glycin,50mM NaCl及20% methanol組成 )下,以水動及電動注射在8分鐘內均可將PQ及DQ分離,在本分析條件下;沒有任何尿液成分會干擾分析。PQ和DQ在未經萃取步驛直接以CZE分析之偵測極限為0.2及0.1 mg/ml,如經固相前處理後再經CZE分析;分析之偵測極限可提高至15及8 ng/ml。CZE在除草劑中毒是一種非常好的一種實驗分析方法。 |
| 英文摘要 | Qualitative and quantitative detection of paraquat and diquat in urine was performed by capillary zone electrophoresis (CZE). In non-extracted urine, these two closely related herbicides were hydrodynamically or electrokinetically injected into the capillary and well separated in a acidic (Ph3.0) buffer (10 mM glycin, 50 mM NaCl and 20% methanol) within 8min. The analytes can be identified by their respective migration time with no interfering endogeous compounds around. The peaks were further confirmed by their respective multi-wavelength scanning spectra. Limits of detection (LOD) were 0.2 and 0.1 mg/ml for PQ and DQ, respectively, in nonex-tracted urine using hydrodynamic injection. Detection sensitivity was greatly improved by solid phase extraction of the compounds followed by CZE using electro-injection under which LDOs were 15 and 8 ng/ml for paraquat and diquat respectively. We conclude CZE is a useful method for laboratory diagnosis of herbicide intoxication. |
本系統中英文摘要資訊取自各篇刊載內容。