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題名 | Generation of Fast-Growing Tilapia by the Application of Yellowfin Porgy Growth Hormone Transgene Which Driven by JAK1 Promoter=以JAK1起動子驅動生長激素基因經由電穿孔精子攜帶轉殖基因製作快速成長的基因轉殖吳郭魚 |
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作者 | 陳昭德; 蔡添財; 賴碩彥; 廖一久; | 書刊名 | 臺灣水產學會刊 |
卷期 | 24:4 1997.12[民86.12] |
頁次 | 頁313-325 |
分類號 | 439.5 |
關鍵詞 | 基因轉殖魚; 全魚轉殖基因; All fish transgene; Transgenic tilapia; |
語文 | 英文(English) |
中文摘要 | 以 JAK1 起動子置於黃鰭鯛生長激素基因( ypGH )之前構建一新轉殖基因載體 pJAKY ,將此轉殖基因以電穿孔法帶入吳郭魚精子內,再以此經由電穿孔過攜有轉殖基因之 精子,與成熟的吳郭魚卵授精,授精卵仍可正常孵化並發育。 自授精起算第 181 天,仍有 14 尾稚魚存活。 自其中 13 尾取胸鰭及腹鰭萃取 DNA 做聚核脢鏈索反應生( PCR )檢測 ,皆証實黃鰭鯛生長激素基因存在魚體細胞內。 體型最小的一尾( 11.72 克)取肝、腦、 肌肉及血液萃取 RNA ,並做反轉錄聚核脢鏈索反應法( RT-PCR )檢測, 結果在肝細胞檢 測出 ypGH mRNA 的存在,並在腦細胞檢測出小量 ypGH mRNA 的存在,顯示轉殖基因確有表 現功能。 但在血液及肌肉則無表現。 在自授精起算第 213 天, 再抽取體型最大的兩尾( 74.79 克及 66.02 克)基因轉殖吳郭魚血做反轉錄聚核脢鏈索反應法檢測, 結果皆檢測出 ypGH mRNA 存在血液中。經越冬蓄養 14 個月後,測量體重做生長指標,以平均體重做比較 ,結果顯示基因轉殖吳郭魚較非基因轉殖吳郭魚成長快四個月。 |
英文摘要 | An "all-fish" transgene, pJAKY was constructed by which consisted of JAK1 promoter and yellowfin porgy growth hormone (ypGH). Tilapia sperm which electroporated with pJAKY was used as plasmids carrier for gene transfer. These electroporated-sperm were immediately mixed with eggs and subsequently added with water to stimulate fertilization. At day 181 since fertilization, 14 presumptive transgenic fishes remained alive. The genomic DNA from 13 fishes of them were extracted from their pectoral and anal fins for PCR analysis. Presence of the integrated transgene within the genome was confirmed by that the expressed ypGH PCR fragments were positively detected from all examined genomic DNA samples. Further, one out of 14 transgenic fish was sacrificed to monitor the expression of the transgene at the transcriptional level by RT-PCR analysis . The expression of ypGH mRNA in the tissue of liver and brain was surely present and could be directly visualized on the agarose gel after electrophoresis. But the ypGH mRNA was absent in the tissue of muscle and blood . During the rearing period after 14 months, the average body weight of those 13 presumptive transgenic fishes was scored by 1.73 folds weightier than that of non-transgenic fishes. Using mean of body weight as parameter for comparison , the group of transgenic fish grow faster than the group of non-transgenic fish around 4 months. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。