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題 名 | Fibroblasts Derived from Tissue Explants of Dilantin-Induced Gingival Hyperplasia and Idiopathic Gingival Fibromatosis Show Distinct Disparity in Proliferative Responsiveness to Epidermal Growth Factor=表皮生長因子對Dilantin促成牙齦增生和自發性牙齦纖維瘤病造纖維細胞增殖之影響 |
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作 者 | 黃景勝; 陳君侃; 陳朝寶; 阮怡仁; 劉燦榮; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 13:10 1997.10[民86.10] |
頁 次 | 頁598-604 |
分類號 | 416.94 |
關鍵詞 | 表皮生長因子; 牙齦增生; 自發性牙齦纖維瘤病; 造纖維細胞增殖; Dilantin-induced gingival hyperplasia; Idiopathic gingival fibromatosis; Epidermal growth factor; |
語 文 | 英文(English) |
中文摘要 | 本研究之目的在探討表皮生長因子(EGF)對造纖維細胞增生之影響。實驗對象:服用Dilantin牙齦增生(DGH)兩位,自發性牙齦纖維瘤病 (GF) 一位,另以健康之牙齦組織 (NG) 為對照。結果顯示,在免疫組織化學染色方面,EGF受器陽性染色細胞,以DGH為最多。在EGF連結實驗方面,雖然DGH細胞之EGF受器為NG與GF之兩倍,但三者對EGF連接之親和力並沒有差異。在EGF剌激細胞增殖方面顯示,DGH細胞隨EGF濃度增加而增加。但NG與GF則無此現象。由上述結果得以下之結論:DGH與GF細胞對EGF之增殖反應不同,顯示此兩種牙齦增生之機轉不同,因此可以預測阻斷EGF刺激造纖維細胞也許可以用來治療DGH。 |
英文摘要 | Human gingival fibroblasts derived from tissue explants or two patients with dilantin-induced gingival hyperplasia (DGH) and one patient with idiopathic gingival fibromatosis (GF) were studied with respect to the effect of epidermal growth factor (EGF) on the proliferative characteristics of these cells. Immunohistochemical staining showed that there were more EGF receptor-positive cells among DGH fibroblasts than among either normal gingival fibroblasts (NG) or GF cells. Furthermore, EGF binding studies showed that, in spite of there being no disparity in binding affinity among all these cells. DGH fibroblasts possessed approximately two-fold more EGF receptors than either NG or GF cells. In addition, the growth- promoting effect of exogenously added EGF was concentration-dependent in DGH fibroblasts but was not in either NG or GF cells. All of the above findings clearly demonstrate that DGH and GF fibroblasts exhibit distinct disparity in proliferative responsiveness to EGF and suggest that different mechanisms may be involved in the pathogenesis of these two forms of gingival hyperplasia. These observations also suggest a possible therapeutic approach for blocking EGF-induced cell proliferation in DGH. |
本系統中英文摘要資訊取自各篇刊載內容。