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題名 | Rapid Detection of Mycobacterium Tuberculosis in Various Clinical Specimens by Using Polymerase Chain Reaction Combined with a Nonradioactive Hybridization System=以聚合酶連鎖反應結合非放射線雜交技術快速診斷臨床檢體中之結核桿菌 |
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作者 | 王聖文; 謝淑鐘; 丁明哲; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷期 | 30:3 1997.08[民86.08] |
頁次 | 頁160-169 |
分類號 | 414.83 |
關鍵詞 | 聚合酶連鎖反應; 非放射線雜交技術; 結核桿菌; Mycobacterium tuberculosis; PCR; DNA hybridization; |
語文 | 英文(English) |
中文摘要 | 本實驗以去氧核醣核酸聚合□連鎖反應(PCR)結合非放射線雜交技術,偵測130個疑似結核病患者臨床檢體中之結核桿菌,共中有62個檢體呈陽性反應;同檢體以傳統培養技術偵測,則只有16個檢體呈陽性;以BACTEC 460放射系統偵測,有18個檢體呈陽性;以抗酸性染色技術檢查,有10個檢體呈陽性;單純以聚合□連鎖反應為判斷標準,有23個檢體呈陽性。如果以培養法為標準鑑定方法,PCR結合非放射線雜交技術的靈敏度及特異性分別為100%(16/16)及59.6(68/114),其特異性雖偏低,但46個培養為陰性而PCR結合非放射線雜交技術偵測為陽性的檢體中,有28個檢體係取自臨床證據顯示為結核病之病人;此外,有四個培養為陰性但BACTEC 460偵測為陽性之檢體,及二個培養為陰性但抗酸性染色檢查為陽性之檢體,以PCR結合非放射線雜交技術偵測均呈陽性。此資料顯示PCR與放射線雜交技術的結合大幅提高了結核桿菌偵測的陽性率及特異性,且只需三天即可完成。若能配合臨床診斷數據並嚴格控制操作過程,PCR結合非放射線雜交技術不失為一良好之篩檢及鑑定技術。 |
英文摘要 | A DNA amplification system using the polymerase chain reaction (PCR) combined with a nonradioactive digoxigenin-labeled probe hybridization was employed to detect Mycobacterium tuberculosis in clinical specimens. One hundred and thirty specimens were tested by several methods including routine culture method, acid-fast staining, BACTEC 460 detection system, PCR, and PCR-hybridization techniques. Sixteen out of 130 specimens were culture positive on Middle-brook 7H11 agar, 10 were positive with acid-fast staining. 18 were positive with BACTEC 460 detection system, 23 were positive with PCR technique, and 62 were positive with PCR-nonradioactive hybridization technique. When compared with culture results. PCR-nonradioactive hybridization had an overall sensitivity of 100 % (16/16) and a specificity of 59.7% (68/114). However, 28 out of 46 (60.9%) PCR-nonradioactive hybridization positive specimens which were culture negative had clinical data supporting thc diagnosis of tuberculosis. In addition, 4 specimens which were negative by routine culture but positive by BACTEC 460 detection system and two specimens which were negative by routine culture but positive by acid-fast staining were all positive by PCR-hybridization technique. These data suggest that routine culture method may not be sensitive enough to detect M. tuberculosis in all kinds of clinical specimens. Taking this deviation into account, the specificity of PCR-nonradioactive hybridization technique may be rectified range from 63% (68/108) to 79.1% (68/86). PCR itself is not satisfactory enough to detect M. tuberculosis in specimens (the sensivitity and specificity were 56.3% and 87.7%. respectively) in this study. However, when it combines with DNA hybridization technique, they can be a very powerful and rapid diagnostic tool to detect M. tuberculosis in clinical specimens. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。