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題 名 | Genetic Localization of the Type I Trimethoprim Resistance Gene and Its Dissemination in Urinary Tract Isolates in Taiwan=泌尿道菌株第一型Trimethoprim抗藥基因存在位置與散佈方式分析 |
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作 者 | 張玲麗; 張榮洲; 張仲羽; 張瑞烽; 吳文正; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 13:9 1997.09[民86.09] |
頁 次 | 頁525-533 |
分類號 | 415.8 |
關鍵詞 | 泌尿道菌株; 抗藥基因; Type I DHFR gene; Tn7; Integron-like structure; |
語 文 | 英文(English) |
中文摘要 | 由泌尿道感染菌株中挑選425株腸內菌,包括E. coli、Enterobacter spp.,及Klebsiella spp.等。對trimethoprim 具有抗藥性者有374株,其中169株(45.5%)攜帶type I DHFR trimethoprim抗藥基因,但在此169株細菌中,發現與Tn7探針雜交者只有17.2%。因此另外探討type I DHFR抗藥基因插入integron之可能性,當取integrase探針進行菌落雜交反應時,結果有87.6%呈陽性反應。進一步經由內核酸鑑識 切割證實type I DHFR抗藥因除了位在轉位子Tn7外,大多數位於integron上。另外,integron於E. coli、Enterobacter spp.及Klebsiella spp.等425株受試之腸內菌中,出現頻率高達86.4%、84.8%及76.7%。可見integron普遍存在這些菌屬間。此外南方雜方反應也指出integron主要位於質體上,因此從本研究中發現在trimethoprim與sulphonamide藥物的雙重選擇壓力下,trimethoprim抗藥基因主要藉著嵌入integron,並靠質體的轉移來傳遞trimethoprim抗藥基因。 |
英文摘要 | In a total of 425 urinary isolates of E. coll, Enterobacter spp., and Klebslella spp. Selected, there were 169 (45.4%) isolates harbouring type I dihydrofolate reductase (DHFR) gene among 374 trimethoprim-resistant isolates. In there 169 isolates, only 17.2% hybridized with the Tn7 probe. According to another probe specific for the integrase gene of integron, 87.6% showed a positive reaction. Further analysis by restriction mapping proved that the type I DHFR gene was inserted into a integron-like structure. These results indicate that the type I DHFR gene that was initially observed in association with transposable element Tn7 is becoming associated with an integrase function similar to integrons in most instances. Further analysis of the distribution of Tn21-like integrase gene in clinical isolates indicated that the prevalence rates were 86.4%, 84.8%, and 76.7% respectively in E. coll, Enterobacter spp., and Klebsiella spp.. Furthermore, the integrase gene found in our clinical isolates proved to be mediated by a plasmid, demonstrated by Southern hybridization. Thus, the trimethoprim-resistant gene that developed under selective pressure from the double drug trimethoprim and sulphonamide was transimitted by insertion into integron-like structure and then mediated by plasmid transfer for dissemination. |
本系統中英文摘要資訊取自各篇刊載內容。