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題 名 | Synergestic Effects of Noise and Aminoglycoside Antibiotic (Gentamicin) on Auditory Function in the Gerbil=噪音和氨基配糖體抗生素 (Gentamicin) 對沙鼠聽覺系統的協同作用 |
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作 者 | 陳贊如; 陳順勝; 林壯澔; 謝悅齡; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 13:7 1997.07[民86.07] |
頁 次 | 頁407-416 |
分類號 | 415.149 |
關鍵詞 | 噪音; 氨基配糖體抗生素; 沙鼠; 聽覺系統; Noise; Aminoglycoside; Gerbil; |
語 文 | 英文(English) |
中文摘要 | 過度噪音曝露後,不論在人類或動物都會造成聽覺損害,而多種不同的耳毒性物質,如氨基配糖體類的抗生素,更會加重其損害程度。在本研究中,由於沙鼠的聽覺功能較其他嘴齒類動物更似於人類,亦即對低頻率的聲音較為敏感,因此選用沙鼠進行動物實驗。以非侵犯性的腦幹聽覺誘發電位記錄(簡稱 BAEPs ) 評估沙鼠在噪音曝露及氨基配糖體注射後的聽覺功能受影響情形。共使用 50 隻雄性成熟沙鼠,將之分為四組,分別為: ( A) 控制組,不曝露噪音或使用氨基配糖體; ( B) 噪音曝露組,每天曝露在 100 分貝噪音下十小時,持續四週; ( C ) 氨基配糖體注射組,每天肌肉注射一劑抗生素( gentamicin 75 mg / Kg),持續四週: ( D) 噪音合併氨基配糖體組,每天同時給予噪音曝露及氨基配糖體注射,持續四週。所有的沙鼠在給予任何實驗處置前 24 小時先記錄一次 BAEPS ,然後每週記錄一次直到第八週為止。結果發現,長期噪音曝露或抗生素注射均造成沙鼠體重減輕。而同時給予噪音及抗生素的處置所造成的聽覺閩值增加比單獨噪音曝露或單獨抗生素注射後各別增加的聽覺閩值總和更大,亦即在噪音及抗生素同時給予的雙重影響下,對聽覺的損害有協同增強作用。不過,四組閒 BAEPs 的平均波潛時及波間潛時並無顯著差異。因此,從本研究結果得知,沙鼠的聽覺閩值明顯升高,顯示噪音或氨基配糖體造成的聽覺損害主要發生在耳蝸,而 BAEPS 潛時僅有輕微改變,顯示對聽覺中樞徑路的損害較小。噪音及氨基配糖體不僅對高頻音的聽覺會有影響,從沙鼠的實驗證明對較低頻率聲音的聽力亦會損害,而該頻率範圍和人類的語言溝通有很密切關係。 |
英文摘要 | Exposure to excessive noise can cause auditory impairment in people as well as in animals, which can be aggravated by many different ototoxic substances, such as aminoglycosides. In the present study, the gerbil was selected as the experimental animal because the gerbil has low-frequency auditory sensitivity that is similar to human beings. Auditory function in gerbils upon exposure to noise and/or gentamicin was evaluated from non-invasive records of brainstem auditory evoked potentials (BAEPs). Fifty mature male Mongolian gerbils were divided randomly into four groups. The control group was exposed neither to noise nor to gentamicin. The noise group was exposed to 100dBA noise ten hours a day for four weeks. The gentamicin group was treated with one dose of gentamicin, 75mg/Kg i.m., daily for four weeks. The noise + gentamicin group was exposed concurrently to both noise and gentamicin treatment. BAEPs were recorded from all gerbils 24 hours before any experimental treatment, then every week for eight consecutive weeks. Long-term exposure to noise and/or gentamicin treatment was found to reduce gerbil’s body weight. The increase in auditory threshold upon concurrent exposure to both noise and gentamicin treatment (evaluated from BAEPs) exceeded the sum of effects caused by exposure to noise alone and to gentamicin alone. Although differences of peak or Interpeak latendes among the four groups were not tatistically significant, large standard deviations were noted in the noise + gentamicin group. Thus the apparent threshold shifts in gerbils indicated a major noise and/or gentamicin induced auditory impairment to the cochlea, and the slight latency variations probably suggested a minor injury to the auditory pathways. We emphasize that noise and aminoglycoside antibiotic gentam1cm can cause damage to the hearing ability at the frequency range vital to human speech recognition, and their effects are synergetic. |
本系統中英文摘要資訊取自各篇刊載內容。