頁籤選單縮合
題名 | Genomic Cloning and Sequence Analysis of Taiwan-3 Human Polyomavirus JC Virus=臺灣三號人類多瘤性JC病毒之基因體選殖及核酸序列分析 |
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作者 | 歐威志; 蔡榮森; 王梅林; 方瓊瑤; 許宗雄; 張德卿; | 書刊名 | 臺灣醫學會雜誌 |
卷期 | 96:7 1997.07[民86.07] |
頁次 | 頁511-516 |
分類號 | 414.84 |
關鍵詞 | 臺灣三號人類多瘤性JC病毒; 基因體選殖; 核酸序列分析; Papovavirus; Taiwan-3 JC virus; Viral genome; Autoimmune disease; |
語文 | 英文(English) |
英文摘要 | Four different strains of human polyomavirus JC virus (JCV), CY, Taiwan-1, Taiwan-2, and Taiwan-3, have been found in pregnant women and autoimmune disease patients in Taiwan. In this study, we report the cloning and sequencing of the Taiwan-3 JCV, virus isolated from the urine of an immunosuppressed patient with rheumatoid arthritis. The viral genome was amplified by polymerase chain reaction and then cloned into a prokaryotic replicative plasmid, pGEM-7Zf(-). The genomic DNA was sequenced and found to comprise 5,111 base pairs. The enhancer-promoter region of the viral genome lacks a copy of pentanucleotide-A (GGGAA) and pentanucleotide-B (AAAGC) compared to the CY archetypal JCV. There are 108 nucleotides altered in the total genome, excluding the variable part of the enhancer-promoter region, between Mad-1 (the prototype JC virus) and Taiwan-3. The enhancer-promoter region has approximately 25% of the altered nucleotides, resulting in amino acid changes in the open reading frames for LT, capsid proteins (VP1, VP2, and VP3), and the agno protein. The cloned Taiwan-3 genome will provide an source for physiologic and pathologic investigation of the JCV virus in the future. |
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