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題名 | Effect of Hormone Treatment on Callus Formation and Endogenous Indole-Acetic Acid and Polyamine Contents of Soybean Hypocotyl Cultivated in Vitro=荷爾蒙對大豆癒合組織之誘發及組織內引朵乙酸和多胺類含量之影響 |
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作者 | 劉景煌; 顏秀芸; 王維章; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 38:3 1997.07[民86.07] |
頁次 | 頁171-176 |
分類號 | 373.1 |
關鍵詞 | 癒合組織; 引朵乙酸; 多胺; Callus; Glycine max; Indole-3-acetic acid; Polyamines; |
語文 | 英文(English) |
中文摘要 | 在MS+NAA+Kinetin培養基上之癒合組織之誘發與生長最好。內生IAA顯著地累積於 MS+NAA+Kinetin處理之下胚軸培植體內,此結果顯示Kinetin可能抑制組織內IAA之分解作用。 於MS+NAA+Kinetin之處理,下胚軸體內所含之多胺類如spermidne及spermine亦明顯增 加,並且此多胺類之增加亦伴隨癒合組織之增長。 |
英文摘要 | Callus was formed and grew well when explants were cultured on medium containing both NAA and kinetin. The endogenous IAA in hypocotyls was accumulated on the medium with both hormones, showing the suppression effect of kinetin on IAA degradation. Besides, the accumulation of NAA in hypocotyl explants was also markedly enhanced by kinetin in the culture medium containing both hormones. In the MS+NAA+Kinetin treatment, the accumulation of spermidine and spermine occurred on the sixth day and was accompanied by rapid callus growth. Increasing putrescine levels were found during the first 3 days in culture when cell multiplication was rapid. The accumulation of endogenous IAA, exogenous NAA, spermidine, and spermine are in proportion to the callus growth in soybean hypocotyl explants. |
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