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題名 | Genetic Variation of Phyllostachys Pubescens (Bambusoideae, Poaceae) in Taiwan Based on DNA Polymorphisms=臺灣產孟宗竹(禾本科竹亞科)之核酸多型性遺傳變異 |
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作 者 | 賴杰治; 蕭如英; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 38:3 1997.07[民86.07] |
頁次 | 頁145-152 |
分類號 | 377.2124 |
關鍵詞 | 孟宗竹; 禾本科; 遺傳變異; 逢機擴增多型性核酸; 微衛星; 迷你衛星; Genetic variation; Microsatellite; Minisatellite; Phyllostachys pubescens; Poaceae; RAPD; |
語文 | 英文(English) |
中文摘要 | 孟宗竹在臺灣為重要經濟竹類,約於十八世紀中葉左右自大陸引進。本研究的目的, 是希望藉核酸之多型性來鑑別臺灣地區現有孟宗竹營養系個數,並研究其分佈的情況。從本 島各地共採得176個樣本,以13個逢機擴增多型性核酸引子和3個微衛星及l個迷你衛星引子來 放大基因組DNA條帶,依據放大的條帶型式來鑑別營養系。結果共鑑定出9個營養系,且由歸 群分析及主座標分析來研究這些營養系間的關係。本種繁衍方式主要依地下莖拓展之無性繁 殖,而本研究結果亦顯示其遺傳變異甚為有限,變異中心在南投縣一帶,含括了所有鑑定出 的9個營養系。而其他地區幾乎是由分佈最廣之單一營養系所構成。此種結果指出南投一帶可 能是最早栽植成功的地區,之後方才引植至其他地區。 |
英文摘要 | Phyllostachys pubescens is an economically important bamboo species in Taiwan. It was introduced from mainland China probably around the middle of the eighteenth century by immigrants. The purpose of the present study is to identify clones and to study clonal distribution in Taiwan based on DNA polymorphisms. One hundred and seventy-six samples were collected around the island. Genomic DNA fragments were amplified using 13 RAPD primers. 3 microsatellite primers, and 1 minisatellite primer. The resulting amplification patterns were used for clone identification. Nine clones were identified from these samples. The relationship between these clones was studied using cluster analysis and principle co-ordinate analysis. The species is reproduced mainly by the rapid spreading of its rhizomes. The results of the present study revealed that its genetic variation is very limited. The center of variation is in Nantou County. The region around Nantou County consisted of all of the nine identified clones while the remaining regions generally consisted of only one common clone. This evidence indicated that the Nantou region might be the first area of successful introduction and that the cultivation later spread to other regions. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。