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頁籤選單縮合
題名 | 應用生物技術保存作物種原多樣性=Applying Biotechnology in Maintaining Plant Germplasm Diversity |
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作者 | 邱輝龍; 范明仁; 張淑芬; | 書刊名 | 中華民國作物種原簡訊 |
卷期 | 2:1 1997.05[民86.05] |
頁次 | 頁8-10 |
分類號 | 434.28 |
關鍵詞 | 生物技術; 作物種原; |
語文 | 中文(Chinese) |
英文摘要 | To maintain plant germplasm diversity, several biotechniques, in broader sense, are applied. For vegetatively propagated crops and those crops whose seeds are recalcitrant, there are a number of potential advantages in conserving them as in vitro culture. These include economics in space and labor if appropriate conservation methods are chosen. The disadvantages include that culture maintenance and the full exploitation are impeded if a species (such as coconut) can not be propagated from cultured tissue or cell, or in conserving woody species because of difficulties in the culture and regeneration of woody species. Two approaches to in vitro storage have been proved successful, namely slow growth media and cryopreservation. The slow growth media could greatly reduce the time, labor, and materials required to maintain the cultures. But it often results in somatic mutation and failure to conserve the genetic integrity of the original clonal material. Cryopreservation offers the prospect of storage for indefinite periods with minimal risk. However, certain cultures can suffer damage during freezing and thawing. Although vitrification and encasing tissue in an alginate gel have been developed to improve, many technical barriers remain, preventing their routine use for plant germplasm conservation. Within the past decade, the rapid progress of molecular techniques have been made and these techniques are considered to be new approaches for preserving germplasm resources. As the DNA sequences in the genomes of germplasm accession are the source of the genes required in breeding program, the conservation of DNA and assembly of sequence data bases are proposal, but others doubt the possibility. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。