頁籤選單縮合
題 名 | 蘇力菌殺蟲晶體蛋白基因轉移至甘藍之研究=Transfer of the Insecticidal Crystal Protein Gene of Bacillus Thuringiensis into Cabbage (Brassica Oleracea L. var. Capitata L.) |
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作 者 | 張隆武; 曾夢蛟; | 書刊名 | 興大園藝 |
卷 期 | 22:1 1997.06[民86.06] |
頁 次 | 頁61-73 |
分類號 | 435.233 |
關鍵詞 | 蘇力菌; 蘇力菌殺蟲晶體蛋白基因; 基因轉移; Bacillius thuringiensis; CrylA(b); Gene transfer; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗將攜帶有Cauliflower Mosaic Virus 35S (CaMV 35S)及rubisco small subunit (rbcS) 啟動子的轉殖載體,以蘇力菌殺蟲晶體蛋白基因 (cry1A(b)), 利用農桿 菌基因轉移法將其轉移到甘藍 (新豐、初秋 ) 的子葉或下胚軸。 本實驗之目的在建立甘藍 基因轉移及植株再生系統,研究不同啟動子對表現基因的影響,並探討培育成抗蟲之蔬菜的 可行性。試驗結果顯示,以農桿菌轉殖兩個品種的甘藍再生率在 2.1% ~3.6% 之間。再生植 株經 PCR 反應作初步篩選後,以南方墨點雜交法分析檢驗,可在轉殖植株的 DNA 上偵測到 雜交訊號。北方墨點雜交分析的結果顯示,存在轉殖植株內的蘇力菌殺蟲晶體蛋白基因可轉 錄出 RNA。 轉殖攜帶 rbcS 啟動子質體的轉殖再生植株, 其 RNA 表現量與轉殖攜帶 CaMV 35S 啟動子質體的植株, 均相似。 經 cry1A(b) 基因轉移之植株的生物檢定結果顯示, 以 rcbS 啟動子為轉殖質體的植株,殺小菜蛾效果較以 CaMV 35S 啟動子為轉殖質體的植株高。 |
英文摘要 | This research focuses on the use of cruciferous vegetables as a model system to establish the gene transfer technology, and to study the possibility for improvement of cruciferous vegetable with insect resistance, through the art of genetic engineering. The δ -toxin gene of Bacillus thuringiensis (cryIA(b)) was transfer into hypocotyl and cotyledon of cabbage (New top, K-Y cross) via Agrobacterium mediated transformation. Regenerated plants were obtained after transformation with two kinds of plasmids. The regeneration rate of transformation was 2.1% to 3.6%. The transformed plants were examined by PCR, Southern and Northern blot hybridization. The results indicated that the expression of constructed genes was a little higher in transgenic plants transferred with rbcS as promoter than CaMV 35S promoter. Insecticidal effects on Plutella xylostellac was demonstrated in cry1A(b)-transformed cabbage plants. |
本系統中英文摘要資訊取自各篇刊載內容。