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題名 | 省產天然臺灣紅豆杉母樹枝葉內紫杉烷類含量之變異=Content Variation of Taxanes in Needles and Stems of Taxus Mairei Trees Naturally Distributed in Taiwan |
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作者 | 何政坤; 張淑華; 陳振榮; Ho, Cheng Kuen; Chang, Shu Hwa; Chen, Zen Zong; |
期刊 | 臺灣林業科學 |
出版日期 | 19970300 |
卷期 | 12:1 1997.03[民86.03] |
頁次 | 頁23-37 |
分類號 | 436.11 |
語文 | chi |
關鍵詞 | 紫杉烷類; 紫杉醇; 臺灣紅豆杉; 變異分析; Taxanes; Taxol; Taxus mairei; Variation analysis; |
中文摘要 | 臺灣紅豆杉以胸徑 50-100 cm 以上的巨樹,稀疏散生於臺灣的高山上。從本省各 地區採集臺灣紅豆杉枝葉 35 株,每株採集小量樣品約 50 g 鮮重, 用 HPLC 分析 6 種紫 杉烷類的含量, 包括: 10-(deacetyl) baccation III (DB), baccatin III (BC), 10-deacetyl taxol (DTX) , cephalomannine (CPH) , taxol (紫杉醇), 7-epi-10-deacetyl taxol (ETX),依各地區、天然群生的種原、母樹進行層級分析, 結果 顯示枝葉中 CPH 與紫杉醇主要的變異來源在地區內種原間, 而 DB、DTX 與 ETX 則來自種 原內母樹間。顯示要找尋高含量紫杉醇化學品系可在地區的種原間發現,而 DB 則可在單株 間發現。 針葉中紫杉烷類間之相關分析顯示,除了 ETX 外,紫杉醇與其他紫杉烷類均呈現 極顯著的相關,但在綠枝中紫杉醇的其他紫杉烷類含量並無相關存在。針葉中紫杉烷類含量 ,除了 DB 與 BC 含量與綠枝相當外,其他均高於綠枝 2-3 倍。 為了進一步由小量樣品確 定紫杉烷類的含量,大量樣品經萃取後經管柱層析純化後再加以定量,結果顯示在管柱的滯 留時間較長的 DTX、CPH,紫杉醇與 ETX 的含量在大、小量樣品間相當一致, 但 DB 與 BC 則顯示被低估。由於不同母樹間針葉中紫杉醇類的含量為 0-368 ppm,DB 為 0-3600 ppm, 二者的變異相當大,顯示其選種價值極高。 |
英文摘要 | Taxus mairei, standing as giant trees with more than 50-100 cm in diame ter at breast height is sparsely scattered in high mountains of Taiwan. To determine content variation of 6 taxanes:10-decaetyl baccatin III (DB), baccatin III (BC) , 10-deacetyl taxol (DTX) , cephalomanine (CPH) , taxol, and 7-epi-10-deacetyl taxol(ETX), small samples (50 mg fresh weight) of needles and stems each were collected separately from 35 individual trees. A-3-level nested analysis was conducted accoring to different locations in Taiwan, the provenance where trees are scattered in the same mountain area, and individual tress within a provenance. The results revaled that most variation of sources of CPH and taxol came from a provenance within locations, whereas that of DB, DTX, and ETX came from individual trees within a provenance for both needles and stems. This suggests that chemical races with high contents of taxol in branches should be selected within a provenance while those with high DB content should be from individual trees. Taxol content in needles showed a very significant relationship with other taxanes except ETX, however, it did not show any relation with other taxanes in stems. Amounts of taxanes in needles were 2-3 times more than those in stems, except for DB and BC. To confirm the amounts of taxanes determined by small-quantiy samples, more precise quantificantion of taxanes was performed by purifying extracts of a large amount of needles using column chromatography. Taxanes with longer retention times such as DTX, CPH, Taxol, and ETX had similar concentrations between small- and large- quantity samples. However, DB and BC, that are easily interfered with impurities, were under-estimated in small samples. In needles of different trees, the existence of great variations of taxol ranging from 0 to 368 ppm and DB ranging from 0 to 3600 ppm showed that it is important to select for breeding in Taxus maire. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。