頁籤選單縮合
題名 | Pituitary Transplantation-A Study on the Response of Hypophysis-Adrenal Axis to Physiological Stress=腦垂腺移植: 腦垂-腎上腺軸反應之研究 |
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作 者 | 王致仁; 洪純隆; 莊銘泉; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 13:2 1997.02[民86.02] |
頁次 | 頁92-96 |
分類號 | 416.291 |
關鍵詞 | 腦垂腺移植; 腦垂-腎上腺軸反應; Pituitary allografts; Pituitary-adrenal axis; |
語文 | 英文(English) |
中文摘要 | 本研究以50隻雄性Sprague-Dawley系老鼠為實驗對象,將40隻摘除腦垂腺,兩週後,以立體定位儀將胎鼠之腦垂腺移植到30隻之第三腦室底部,近下視丘區下的10隻不進行移植作為缺乏腦垂腺之動物,另外10隻未摘除腦垂腺者作為對照組。隨後在相同條件下養5個月,分別在其內頸靜脈置入導管,注入insulin, corticotrophin releasing-hormone(CRH)或vehicle,每隔0,15,30分鐘各抽取一次血液,以RIA方法測定成鼠體內adrenal corticotrophin (ACTH) 及corticosterone之level。實驗結果顯示,經腦垂腺移植的動物在注入CRH後,血液中ACTH及corticosterone明顯上升,和對照組之差異不大。注射insulin誘發的低血糖在對照組中造成ACTH及corticosterone有意義上升,在移植腦垂腺的動物中corticosterone上升,顯示移植胎鼠腦垂腺後可以有接近正常的腦垂-腎上腺軸反應。 |
英文摘要 | Previous studies on experimental intracerebral pituitary transplantation have shown survival and possible function of fetal pituitary glands grafted to the hypothalamic region of inbred strains. In the present study, fetal pituitary tissue was implanted into hypothalamic area of the hypophysectomized rat by stereotactic methods. We investigated the hormonal levels and secretions of fetal pituitaries grafted to the allogenic host and studied the effects of physiological stimuli on hypophysectomized rats with grafted pituitaries. We found that the fetal pituitary allografts released adrenal corticotrophin (ACTH) and corticosterone in response to stimulation by corticotropinreeasing hormone (CRH), and released corticosterone alone in response to insulininduced hypoglycemia. The grafts are acutely responsive to CRH and retain their potential for physiological regulation and function. The ACTH-releasing-signal generated in the host by insulin-induced hypoglycemia failed to activate the graft, so activation by other physiological stimuli cannot ruled out. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。