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題名 | Effect of Tonicity and Additives to the Fixative on Ultrastructure of Mesophyllous Cells in Kandelia Candel (L.) Druce(Rhizophoraceae)=固定液中之滲透勢與添加物種類對水筆仔紅樹林葉肉細胞內之微細構造之影響 |
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作者 | 黃元勳; 陳樹春; Hwang, Yuan-hsun; Chen, Shuh-chun; |
期刊 | Botanical Bulletin of Academia Sinica |
出版日期 | 19970100 |
卷期 | 38:1 1997.01[民86.01] |
頁次 | 頁21-28 |
分類號 | 377.22 |
語文 | eng |
關鍵詞 | 水筆仔; 紅樹林; 滲透勢; 鹽; 蔗糖; 張力; Kandelia candel; Mangrove; Osmotic potential; Salt; Sucrose; Tonicity; |
英文摘要 | 本實驗為瞭解水筆仔微細構造樣品製備過程裡,固定液中滲透勢及添加物對其微 細構造的影響。非離子性添加物如蔗醣較離子性添加物如 NaCl,KCl,Na �� SO �痔� K �� SO �痊假z想。 決定固定液的滲透勢前必須先測試水筆仔葉片的滲透勢,結果顯示等張固定 液及等張磷酸緩衝液加上 0.15M 蔗醣, 可得較理想的結果:即葉綠體與粒線體之外膜既不 見膨脹亦不會萎縮。若以蔗糖改變固定液之滲透勢,只改變葉綠體與粒線體外膜之脹縮,並 不會造成其內部微細構造之變化。然而,以離子性添加物增加固定液的滲透勢,會使葉綠體 之類囊膜構造產生嚴重之膨脹。 其中尤以 NaCl 和 KCl 為甚。這些離子性添加物可能影響 類囊膜構造內部離子分布之平衡。 undergo plasmolysis. Varying the tonicity of the fixative with sucrose to a reasonable degree did not change the ultrastructural details within the chloroplasts and mitochondria. In contrast, hypertonic fixatives obtained by adding ionic additives caused significant swelling of thylakoid membranes in chloroplasts. This artifact was most pronounced when hypertonic fixatives containing NaCl or KCl were used. These observations suggest that high ionic fixatives disturb the ionic balance in thylakoid membranes. |
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