頁籤選單縮合
| 題 名 | 臺灣肖楠組織培養之初步研究=Preliminary Studies on Tissue Culture of Calocedrus Formosana (Florin) Florin |
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| 作 者 | 林志謀; 王亞男; 姜家華; | 書刊名 | 國立臺灣大學農學院實驗林研究報告 |
| 卷 期 | 11:1=215 1997.03[民86.03] |
| 頁 次 | 頁55-72 |
| 分類號 | 436.259 |
| 關鍵詞 | 臺灣肖楠; 組織培養; Calocedrus formosana (florin) florin; Tissue culture; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 本試驗嘗試探討並建立臺灣肖楠（Calocedrus formosana（Florin）Florin）之 組織培養之微體繁殖體系。初步結果：臺灣肖楠表面消毒試驗之結果，種子可達 90 ％之無 污染率， 未成熟胚則可達零污染，4 ∼ 6 個月實生苗莖段可控制在 92.5 ％無污染率。 2 ∼ 3 年生實生苗莖段控制污染僅能達到 57.5 ％之無污染率， 具培植體於消毒後多褐化死 亡。在器官形成方面：成熟胚培植於 WPM、MS、B �N三種基礎培養基對無菌苗之誘導效果最 好。 未成熟胚培養於添加 600 mg/l casein hydrolysate、500 mg/l yeast extract、0.5 mg/l BA 及 1 mg/l NAA 之 WPN 培養基中，可誘導出多芽體。無菌苗帶節莖段在添加 0.05 mg/l NAA 與 0.5 mg/l BA 之 WPM 培養基中可誘得最多腋芽之發生。4 ∼ 6 個月實生苗針 葉帶節莖段在含有 0.05 mg/l NAA 及 1 mg/BA 處理可誘導腋芽之發生， 鱗狀葉培養於 1 mg/l BA 與 0.05 mg/l NAA 之 WPM 培養基中可誘導形成腋芽，但芽體叢生狀並玻璃質化， 而在含有 0.01 mg/l BA 與 0.05 mg/l TDZ 之處理可獲得 60 ％之不定芽誘導率。 癒合組 織在含有 1 mg/l NAA 及 0.1 mg/l BA 之 MS 培養基誘導效果最佳。 |
| 英文摘要 | This report describes the development of in vitro culture method for Calocedrus formosana (Florin) Florin. The primary results are summarized as follow. Mature embryos, immature embryos, needle-like leaves and scale-like leaves from 4-6 month-old seedlings, and shoot tips from lateral branchesof 2-3-year-old trees were used as explants. In surface sterilization, immature embryos were decontaminated completely. Ninety percent of the seeds were sterilized. Explants from 4 to 6-month-old seedlings were 92.5 ％ sterile. Although 57.5 ％ of the explants derived from 2 to 3-year-old trees were decontaminated, none of the explants survived in the subsequent culture. The MS, WPM and B �N were the three best media for raising sterile plants. In vitro multiple shoots were successfully induced from immature embryos cultured on the WPN medium containing 600 mg/l casien hydrolysate, 500 mg/l yeast extract, 0.5 mg/l BA and 1 mg/l NAA. Bud formation was optimal on WPN medium supplemented with 0.05 mg/l NAA and 0.5 mg/l BA when nodal segments from sterile seedligs were cultured. Needle-like leaves with nodal segments excised from 4 to 6-month-old seedlings could induced bud formation when culturing on WPN medium containing 0.05 mg/l NAA and 1 mg/l BA. Although bud formation could also obtained from scale-like leaves cultured on WPN medium containing 1 mg/l BA and 0.05 mg/l NAA, buds were clumped and vitrified. However, bud formation was increased to 60 ％ if scale-like leaves were cultured on WPN medium supplemented with 0.01 mg/l BA and 0.05 mg/l TDZ. Callus induction was optimal on MS medium containing 1 mg/l NAA and 0.1 mg/l BA. |
本系統中英文摘要資訊取自各篇刊載內容。