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題 名 | 腸炎弧菌No.93之溶血反應特性=Characterization of Haemolysis of the Vibrio Parahaemolyticus No.93 |
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作 者 | 蘇訓正; 李佳音; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷 期 | 30:1 1997.02[民86.02] |
頁 次 | 頁32-42 |
分類號 | 414.83 |
關鍵詞 | 腸炎弧菌; 溶血反應; |
語 文 | 中文(Chinese) |
中文摘要 | 腸炎弧菌(Vibrio parahaemolyticus)為感染型食物中毒細菌,此菌所產生之溶血素(haemolysin)被認為是重要的致病因子之一。為了解本地區分離之腸炎弧菌所產生之溶血素之特性,以期能更深入解析此溶血素之毒性效能及其致病機轉,乃進行本項研究。其中一株由臨床檢體來的腸炎弧菌No.93菌株可在7% NaCl Wagatsuma medium含血培養基上產生弱溶血現象,但由核酸雜交反應判斷此菌株所帶之溶血素基因異於tdh或trh基因。腸炎弧菌No.93在含人血之3% NaCl Wagatsuma培養基生長時有明顯之溶血現象。粗純化腸炎弧菌No.93之胞外蛋白質,以培養基培養法分析,測試其溶血反應之耐熱性與酵素活性,結果顯示No.93之溶血反應屬熱不穩定型。而從10% SDS-PAGE蛋白質電泳分析發現腸炎弧菌No.93之粗純化胞外蛋白質於64 kDa處有一明顯的蛋白質產物。另外,由gelatin-SDS-PAGE及 hemoglobin-SDS-PAGE活性電泳分析中,得知腸炎弧菌 No.93 之粗純化胞外蛋白質在62 kDa、52 kDa、41 kDa位置處均顯現溶斑,藉此推斷,腸炎弧菌No.93之粗純化胞外蛋白質中含有可以gelatin為反應受質且可分解血紅素之蛋白�t。此蛋白�t是否為造成腸炎弧菌No.93之溶血現象的成因之一,則需進一步的進行蛋白質純化及基因選殖之工作以便證實。 |
英文摘要 | vibrio parahaemolyticus is a causative bacterium of food poisoning, and the haemolysin produced by this organism has been considered as one of the important virulence factors. In order to understand the pathogenic mechanism of this bacterium, the characteristics of haemolysin from Vibrio parahaemolyticus isolated from Taiwan were studied. One of the clinical strains, V. parahaemolyticus No.93, presents a weak hemolytic zone on 7% NaCl-wagatsuma medium. The DNA hybridization results show that V. parahemolyticus has neither tdh nor trh gene. V. parahaemolyticus No.93 shows obviously hemolytic zone on 3%-NaCl Wagatsuma medium (human blood). The crude extracellular protein of V. paraemolyticus No.93 was evaluated for its heat tolerance and enzyme activities by media assay. The results show that this crude extracellular protein is thermolabile. the crude extracellular protein of V. parahaemolyticus No.93 was analyzed on 10% SDS-PAGE and an apparent band of 64 kDa protein was observed. Furthermore, the crude extracellular protein was analyzed by running gelatin -SDS-PAGE and hemoglobin -SDS-PAGE, and three clear zones on 62 kDa, 52kDa and 41kDa were observed on both SDS-PAGEs. Thus we propose that the crude extracellular protein of the V. parahaemolyticus No.93 can degrade gelatin as well as hemoglobin. whether these protease being the virulence factors of Vibrio parahaemolyticus No.93 needs to be further studied. |
本系統中英文摘要資訊取自各篇刊載內容。