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題名 | An Analysis of SELCON--A Self-Consistent Method That Estimates Protein Secondary Structure from Circular Dichroism Data=SELCON, 一個以圓二色數據來估計蛋白質二級結構的程式的分析研究 |
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作者 | 林維政; 甘魯生; | 書刊名 | 中央研究院化學研究所集刊 |
卷期 | 44 1997.03[民86.03] |
頁次 | 頁45-59 |
分類號 | 346.77 |
關鍵詞 | 圓二色數據; 蛋白質; 二級結構; |
語文 | 英文(English) |
英文摘要 | 蛋白質之所以有功能是它們是氨基酸鏈可以折疊成一定的構造,圓二色是在水溶 液中測量蛋白質二級結構最方便的方法,唯一的缺點是一種不自我矛盾的方法,所以二十年 來科學致力於尋求更完美的自我標準。Sreerama 和 Woody 二氏所完成之 SELCON 程式是近 年來比較完善的, 本篇的目的是將 SELCON 仔細加以分析, 將所有參數加以比較之後發現 SELCON 確是一有用之程式, 因此之故我們也改良了此程式數數輸入方式, 使利用 SELCON 時更加方便及快速。這個輸入之程式也收錄在附錄中。 tiplication factor (f) in the calculation is found between 0.95 and 1.05. Second, all three data bases (Kabsch/sander [Biopolymers, 1983, 22, 2577] [KS], Levitt/Greer [J. Mol. Biol., 1977, 114, 181][LG]. and Hennessey/Johnson [Biochemistry, 1981, 20, 1085][HJ] used in the program are good for correlating the estimed and found structures in globular proteins. third, the estimation of the percentages of B-sheets is slight off for all three data bases. Fourth, there is a tendency to overestimate for both the a-helix and b-sheed of a protein Finally, the format of input of the program was revised to be user friendly. A demonstration is collected as an Appendix. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。