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題名 | A Practical Method for Direct Measurement of Prothrombin Time and Activated Partial Thromboplastin Time in Whole Blood=全血凝血酶原時間和活化部份凝血活酶時間之直接測定法 |
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作者 | 陸為群; 江慶弘; 李正華; | 書刊名 | 醫學研究 |
卷期 | 17:2 1996.09[民85.09] |
頁次 | 頁100-106 |
分類號 | 414.81 |
關鍵詞 | 凝血酶原時間; 活化部份凝血活酶時間; 口服抗凝血劑治療; Prothrombin time; Activated partial thromboplastin time; Oral anticoagulant therapy; |
語文 | 英文(English) |
英文摘要 | A novel method proposed for direct measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) in blood, instead of in plasma, is described. PT and APTT are the two parameters most frequently used I clinic. Particularly in this respect are in the course of the monitor anticoagulant therapy and the pre-surgery screenings on the coagulation function. In this investigation we have studied 376 cases, 66 of them were taking coumadin as the oral anticoagulant. The value of PT in the whole blood and plasma, determined with a KC-40 instrument, were 13.92±0.07 and 12.48±0.49 sec, respectively, in the individuals without taking anticoagulants while those of APTT were 39.32±4.63 and 31.14±3.03 sec, respectively. After converting PT to PT ratio ® or International Normalized Ratio (INR), the correlation coefficient (r) was found to be 0.99. The hemocrit (Hct) values ranged from 25.6% to 52.4%. We found that, between the whole blood and plasma, there was no significant difference either in the PT or APTT ratios (p>0.05, null hypothesis accepted). Our findings proved to be highly applicable: the direct measurement of PT and APTT in blood gave excellent results and provided a simple, rapid, and efficient method for routine analysis and their data can be readily converted to the corresponding values in plasma after linear regression analysis. |
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