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相關文獻
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頁籤選單縮合
題 名 | Polymerase Chain Reaction Analysis for Viral Nucleic Acids in Acute Sporadic Hepatitis Patients Negative for Serum Hepatitis B Surface Antigen and Antibodies to Hepatitis C Virus=以聚合酶鏈反應分析血清B型肝炎表面抗原及C型肝炎抗體呈陰性之急性散發性肝炎病人之病毒核酸 |
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作 者 | 霍德義; 吳肇卿; 顏福順; 沈一錚; 李壽東; | 書刊名 | 中華醫學雜誌 |
卷 期 | 58:6 1996.12[民85.12] |
頁 次 | 頁379-384 |
分類號 | 415.5332 |
關鍵詞 | B型肝炎病毒; C型肝炎病毒; E型肝炎病毒; 核酸; 聚合媒鏈反應; Hepatitis B virus; Hepatitis C virus; Hepatitis E virus; Nucleic acid; Polymerase chain reaction; |
語 文 | 英文(English) |
中文摘要 | 背景 聚合(脢)鏈反應的分析對於釐清各種病毒性肝炎之病因是一極 為敏感而且重要的方法。 方法 本篇報告係以此法來研究連續33位病人傳統血清學方法診斷為急性散 發性非A非B非C型肝炎之病毒核酸,並探討其與預後的關係。 結果 共有10位 (30.3%) 病人可在其血清中測得病毒核酸。在27位肝炎病情 自然緩解的病人中,HBV DNA及HCV RNA各有2位 (7.4%) 可測得。相反的, 在6位形成慢性肝炎的病人中,有2位 (33.3%, p = 0.14) 可測得HBV DNA,有 3位 (50%, p = 0.03) 可測得HCV RNA;另有一位可同時測得HBV DNA及HCV RNA。所有病人均無法測得得HDV RNA。雖然有4位病人 (12.1%) 可測得anti- HEV,但是HEV RNA均無法測得。肝炎病情能自然緩解的情形多發生在無法測 得病毒核酸的病人身上(p < 0.01)。 結論 本結果顯示對於診斷某些急性散發性HBsAg及anti-HCV均呈陰性之肝 炎,血清學及分子生物學的檢查具有不可或缺的互補效果。另外,新近發現的 GB肝炎病毒在那些病因仍然不清楚的肝炎,其所扮演的角色亦值得進一步探 討。 |
英文摘要 | Background. Polymerase chain reaction (PCR) is both very sensitive and very valuable in the clarification of the pathogenesis of viral hepatitis from type A to E (HAV to HEV). Methods. This study was aimed to detect viral nucleic acids with PCR in 33 consecutive, acute sporadic hepatitis patients who were seronegative for hepatitis B surface antigen and antibody to hepatitis C virus by conventional radioimmunoassay or enzyme-linked immunoassay. Results. Of the totals, 10 (30.3%) had detectable viral genomes. HBV DNA and HCV RNA were each detected in 2 (7.4%) of 27 patients with a self-limiting course. By contrast, HBV DNA was detected in the two (33.3%, p = 0.14) and HCV RNA in the three (50%, p = 0.03) of the six patents who became chronic; another one who had subsequent multiple exacerbations of hepatitis was positive for both HBV DNA and HCV RNA. HDV RNA was not detectable in all subjects. Although four (12.1%) were positive for antibody against HEV, none had detectable HEV RNA. Spontaneous disease resolution predominantly occurred in patients without detectable hepatitis B and hepatitis C genomes (100% of 23 patients vs 40% of 10 patients, p < 0.01). Conclusions. These results demonstrate that a combination of serological and molecular tests is mandatory for the appraisal of acute sporadic non-B non-C hepatitis and its clinical prognosis, they also raise the possibility of a hepatotrophic agent other than HAV to HEV. Recent documentation of the new GBV-C (hepatitis G virus) suggests the necessity of studying the unidentified pathogenesis in patients with non-A to E hepatitis. |
本系統中英文摘要資訊取自各篇刊載內容。