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相關文獻
- Detection of IgM Antibody to Japanese Encephalitis Virus Infection by Enzyme-Linked Immunosorbent Assay (ELISA)
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- 臺灣北中南東四區山地和平地鄉3-6歲兒童日本腦炎病毒感染的血清流行病學研究
- 酵素免疫吸附法檢測日本腦炎中和抗體之研究
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題 名 | Detection of IgM Antibody to Japanese Encephalitis Virus Infection by Enzyme-Linked Immunosorbent Assay (ELISA)=酵素免疫吸附法檢測日本腦炎病例IgM抗體 |
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作 者 | 周玲; 樂怡雲; 黃耀雄; 林翠莉; 吳盈昌; 洪其璧; | 書刊名 | 中華醫學雜誌 |
卷 期 | 58:1 1996.07[民85.07] |
頁 次 | 頁1-6 |
分類號 | 415.235 |
關鍵詞 | 日本腦炎IgM抗體; 日本腦炎病毒感染; 酵素免疫吸附法; JE-IgM; JEV infection; MAC-ELISA; |
語 文 | 英文(English) |
中文摘要 | 背景 日本腦炎為台灣地區重要疾病之一,每年流行期由正月到九 月,但全年均可看到病例。過去十年來,每年均有二百餘報告病例,其中確定 病例十到三十餘例。由於很難自病人分離到日本腦炎病毒,因此實驗室的檢驗 以血清學為主如血球凝集抑制試驗 (HI) 及中和試驗 (NT) ,但因HI試驗須有 急性期與恢復期兩次血清比較才能判定結果,而NT方法耗時且費力。由於目 前市面上仍無商品化之日本腦炎IgM抗體酵素兔疫吸附檢驗試劑。本計畫擬發 展一敏感,快速之酵素免疫吸附檢定法檢測日本腦炎IgM抗體,作為日本腦炎 近期感染之指標。 方法 收集經HI確認之日本腦炎陽性及陰性血清檢體,以酵素兔疫吸附法測 其日本腦炎IgM抗體。試劑中所使用之生物素-抗體及抗生物素-山葵過氧化 (脢)結合體之最適濃度分別為1:2000及1:15000。 結果 由1987至1989年經HI確定為日本腦炎病例之59對配對血清檢體,以 酵素免疫吸附法測其日本腦炎IgM抗體,該抗體之陽性率自發病後第一星期內 至第四星期分別為65.7% (25/38),73.9% (17/23),93.5% (29/31)及88.8% (8/9)。自 發病日後第五星期至第十星期採血的17個血清檢體則IgM抗體100%呈陽性。 1994年13例確定病例有84.6%(11/13)第一次採急性期血清即可測得JE-IgM。 結論 由於日本腦炎病人在臨床症狀出現時病毒血症期業已消失,因此無法分 離到病毒。急性期病人在發病後一星期內日本腦炎IgM抗體有65.7%呈陽性, 隨著發病日之增加,IgM抗體之陽性率亦隨著提高。日本腦炎IgM抗體之出現 可以當作近期感染的指標,作為快速診斷方法之一。 |
英文摘要 | Background. Japanese encephalitis (JE) is an important infectious disease in Taiwan, with reported cases observed all the year around. Laboratory tests for JE consist mainly of hemagglutination inhibition (HI) test and neutralization test (NT). Commercialized enzyme-linked immunosorbent assay (ELISA) kits for detection of JE-IgM are still not available. Therefore an attempt has been made to develop a sensitive and rapid ELISA for detection of the IgM antibody to JE to serve as an indication of recent Japanese encephalitis virus infection. Methods. Both positive and negative JE serum specimens, confirmed by HI test, were checked for IgM antibody to JE by ELISA. The optimum concentration of biotin-IgG and avidin horse-radish peroxidase conjugate used in MAC-ELISA were 1/2000 and 1/ 15000, respectively. Results. From 1987 to 1989, 118 paired serum specimens of HI-confirmed JE patients were tested for JE-IgM by ELISA. The positive rate of JE-IgM was 65.7% (25/38), 73.9% (17/23), 93.5% (29/31) and 88.8% (8/9) for the consecutive first to fourth weeks after onset of the disease. The JE-IgM antibody of 17 serum specimens collected from the 5th to the 10th week after onset of the disease were 100% detected. In addition, among the 13 HI-confirmed JE cases occurring in 1994, 84.6% of the acute phase serum specimens demonstrated the JE-IgM antibody. Conclusions. About 65.7% of the JE-IgM of the acute serum specimens collected within one week after onset of the disease were detected. The JE-IgM positive rate elevated as the days from disease onset increased. Therefore the appearance of JE- IgM could be used as an indication of recent JEV infection to serve as a rapid laboratory diagnostic tool. |
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