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題名 | Analysis of Genetic Diversity of Two Intersterility Groups of Ganoderma Australe by DNA Sequencing=利用DNA定序法探討南方靈芝兩群雜交不孕性的遺傳變異 |
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作者姓名(中文) | 張君玉; 葉增勇; 李桂楨; | 書刊名 | 師大生物學報 |
卷期 | 31:1 1996.06[民85.06] |
頁次 | 頁47-53 |
分類號 | 379.1 |
關鍵詞 | 南方靈芝; 雜交不孕性群; 內部轉錄間隔片段; DNA定序法; Ganoderma australe; Intersterility group; Internal transcribed spacer; DNA sequencing; |
語文 | 英文(English) |
中文摘要 | 本研究在以聚合酶鏈反應(PCR)和DNA定序法(DNA sequencing),探討南方靈芝Ganoderma australe (Fr.) Pat.種內的兩群雜交不孕性(intersterility group)的遺傳變異,並以不同亞屬的新日本靈芝G. neojaponicum lmaz.作為比較。以互補於酵母茵的rRNA基因的引子與DNA聚合酶,將rRNA基因重覆單位的內部轉錄間隔片段(ITS1、ITS 2)選殖出來。置入pGEM-T載體後,將篩選出的純系進行DNA序列分析。 再以PAUP軟體,分析各樣品ITS1、ITS 2序列的歧異度,建築演化樹狀圈。我們的結果顯示南方靈芝種內的兩群雜交不孕性已呈現顯著的遺傳分化。 |
英文摘要 | Genetic diversity of two intersterility groups of Ganoderma australe (Fr.) Pat. (subgenus Elfoingia) was investigated by polymerase chain reaction (PCR) and DNA sequencing. G. neo-japonicum Imaz. (subgenus Ganoderma) was selected for comparative studies. The DNA fragments containing internal transcribed spacer (ITS) regions were amplified by using oligonucleotide primers complementary to rRNA genes of Saccharomyces cerevisiae. The PCR-amplified products were subcloned into pGEM-T vector and sequenced. Phylogenic structures constructed from analyses of ITS regions suggest that two intersterility groups of G. australe are genetically differentiated. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。