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題名 | 尿中[fed0]-ALA之螢光法測定值與血中鉛含量之相關研究=Studies of Fluorometric Determination of Urinary δ-ALA Concentration and Its Correlation with Blood Lead Content |
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作者姓名(中文) | 劉麗寶; | 書刊名 | 大仁學報 |
卷期 | 14 1996.03[民85.03] |
頁次 | 頁55-67 |
分類號 | 412.53 |
關鍵詞 | 血中鉛; 尿; 胺基左糖酸; 乙酼丙酮; 甲醛; 螢光誘導體; Blood lead; Urine; [fed0]-ALA; Acetylacetone; Formaldehyde; Fluorescent derivative; HPLC; |
語文 | 中文(Chinese) |
中文摘要 | 鉛中毒診斷的指標之一尿中δ-ALA(胺基左糖酸)的螢光定量法已被成功地開發出來了。將尿檢體與acetylacetone(乙醯丙酮)與formaldehyde(甲醛)於沸水中加熱反慹,使其生成具特異性之螢光誘導體,經HPLC(高速液體層析儀)的ODS Column(YMC-PACK)分離,由螢光測定器而得到高感度、良好直線性及再現性的結果。以此法測定110名鉛作業勞工的平均尿中δ-ALA排泄量為3.63 ± 8.05mg/L(3.65 ± 6.25mg/g creatinine)(血中鉛含量為50.8 ± 21.6μg/dl),非鉛作業勞工55 名的平均尿中δ-ALA 排泄量為0.91 ± 0.37mg/L(0.43 ± 0.24mg/g creatinine)(血中鉛含量為9.9 ± 4.2μ g/dl)。這種具簡易、高感度與快速諸優點的定量法,在運用於勞工的健康管理上,對工業衛生的鉛暴露診斷與評估將有很大的貢獻。 |
英文摘要 | Urinary δ-ALA (ALA-U) is one of effective diagnostic index for early lead poisoning. It has been determined usually by the Marzerall-Granick's method using column of Dowex 2 resin in the acetate form or the Tomokuni's method using the extration with the organic solvent. Recently, a highly sensitive and simple method for determining ALA-U using reversed-phase high performance liquid chromatography (HPLC) is described. A fluorescent derivative with a specific character was derived from ALA-U by reaction with acetylacetone and formaldehyde in boiling water bath. It can be well separated through a ODS column (YMC-PACK) by HPLC. Quantative results were obtained when (0.5-30.0 mg/L) δ-ALA standard solutions were tested (r=0.999). Sample recovery for 10 urine samples was about 96.7%. The concentration of δ-ALA in urine for 110 lead workers were 3.63 ± 8.05mg/L and corrected by creatinine to 3.65 ± 6.25 mg/g creatinine while blood content were 50.8 ± 21.6 μg/dl. The concentration of urinary δ- ALA for 55 controls were 0.91 ± 0.37 mg/L and corrected by creatinine to 0.43 ± 0.24 mg/g creatinine while blood content were 9.9 ± 4.2μ/dl.The advantages of simple procedure, high sensitivity and rapidity make this method useful for screening lead workers. |
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